IGDRPT (Indian Genetic Disease Risk Prediction Tool)

Disease Name: Achondroplasia

1 .Inheritance

Autosomal Dominant

2. Gene Name

FGFR3

3. Protein Name

Fibroblast growth factor receptor 3

4. Chromosomal Location

4p16.3

5. Protein Sequence

>NP_000133.1 fibroblast growth factor receptor 3 isoform 1 precursor [Homo sapiens]

MGAPACALALCVAVAIVAGASSESLGTEQRVVGRAAEVPGPEPGQQEQLVFGSGDAVELSCPPPGGGPMG

PTVWVKDGTGLVPSERVLVGPQRLQVLNASHEDSGAYSCRQRLTQRVLCHFSVRVTDAPSSGDDEDGEDE

AEDTGVDTGAPYWTRPERMDKKLLAVPAANTVRFRCPAAGNPTPSISWLKNGREFRGEHRIGGIKLRHQQ

WSLVMESVVPSDRGNYTCVVENKFGSIRQTYTLDVLERSPHRPILQAGLPANQTAVLGSDVEFHCKVYSD

AQPHIQWLKHVEVNGSKVGPDGTPYVTVLKTAGANTTDKELEVLSLHNVTFEDAGEYTCLAGNSIGFSHH

SAWLVVLPAEEELVEADEAGSVYAGILSYGVGFFLFILVVAAVTLCRLRSPPKKGLGSPTVHKISRFPLK

RQVSLESNASMSSNTPLVRIARLSSGEGPTLANVSELELPADPKWELSRARLTLGKPLGEGCFGQVVMAE

AIGIDKDRAAKPVTVAVKMLKDDATDKDLSDLVSEMEMMKMIGKHKNIINLLGACTQGGPLYVLVEYAAK

GNLREFLRARRPPGLDYSFDTCKPPEEQLTFKDLVSCAYQVARGMEYLASQKCIHRDLAARNVLVTEDNV

MKIADFGLARDVHNLDYYKKTTNGRLPVKWMAPEALFDRVYTHQSDVWSFGVLLWEIFTLGGSPYPGIPV

EELFKLLKEGHRMDKPANCTHDLYMIMRECWHAAPSQRPTFKQLVEDLDRVLTVTSTDEYLDLSAPFEQY

SPGGQDTPSSSSSGDDSVFAHDLLPPAPPSSGGSRT

6. Gene Sequence

>NM_000142.5 Homo sapiens fibroblast growth factor receptor 3 (FGFR3), transcript variant 1, mRNA

AGTGCGCGGTGGCGGCGGCGTCGCGGGCAGCTGGCGCCGCGCGGTCCTGCTCTGCCGGTCGCACGGACGC

ACCGGCGGGCCGCCGGCCGGAGGGACGGGGCGGGAGCTGGGCCCGCGGACAGCGAGCCGGAGCGGGAGCC

GCGCGTAGCGAGCCGGGCTCCGGCGCTCGCCAGTCTCCCGAGCGGCGCCCGCCTCCCGCCGGTGCCCGCG

CCGGGCCGTGGGGGGCAGCATGCCCGCGCGCGCTGCCTGAGGACGCCGCGGCCCCCGCCCCCGCCATGGG

CGCCCCTGCCTGCGCCCTCGCGCTCTGCGTGGCCGTGGCCATCGTGGCCGGCGCCTCCTCGGAGTCCTTG

GGGACGGAGCAGCGCGTCGTGGGGCGAGCGGCAGAAGTCCCGGGCCCAGAGCCCGGCCAGCAGGAGCAGT

TGGTCTTCGGCAGCGGGGATGCTGTGGAGCTGAGCTGTCCCCCGCCCGGGGGTGGTCCCATGGGGCCCAC

TGTCTGGGTCAAGGATGGCACAGGGCTGGTGCCCTCGGAGCGTGTCCTGGTGGGGCCCCAGCGGCTGCAG

GTGCTGAATGCCTCCCACGAGGACTCCGGGGCCTACAGCTGCCGGCAGCGGCTCACGCAGCGCGTACTGT

GCCACTTCAGTGTGCGGGTGACAGACGCTCCATCCTCGGGAGATGACGAAGACGGGGAGGACGAGGCTGA

GGACACAGGTGTGGACACAGGGGCCCCTTACTGGACACGGCCCGAGCGGATGGACAAGAAGCTGCTGGCC

GTGCCGGCCGCCAACACCGTCCGCTTCCGCTGCCCAGCCGCTGGCAACCCCACTCCCTCCATCTCCTGGC

TGAAGAACGGCAGGGAGTTCCGCGGCGAGCACCGCATTGGAGGCATCAAGCTGCGGCATCAGCAGTGGAG

CCTGGTCATGGAAAGCGTGGTGCCCTCGGACCGCGGCAACTACACCTGCGTCGTGGAGAACAAGTTTGGC

AGCATCCGGCAGACGTACACGCTGGACGTGCTGGAGCGCTCCCCGCACCGGCCCATCCTGCAGGCGGGGC

TGCCGGCCAACCAGACGGCGGTGCTGGGCAGCGACGTGGAGTTCCACTGCAAGGTGTACAGTGACGCACA

GCCCCACATCCAGTGGCTCAAGCACGTGGAGGTGAATGGCAGCAAGGTGGGCCCGGACGGCACACCCTAC

GTTACCGTGCTCAAGACGGCGGGCGCTAACACCACCGACAAGGAGCTAGAGGTTCTCTCCTTGCACAACG

TCACCTTTGAGGACGCCGGGGAGTACACCTGCCTGGCGGGCAATTCTATTGGGTTTTCTCATCACTCTGC

GTGGCTGGTGGTGCTGCCAGCCGAGGAGGAGCTGGTGGAGGCTGACGAGGCGGGCAGTGTGTATGCAGGC

ATCCTCAGCTACGGGGTGGGCTTCTTCCTGTTCATCCTGGTGGTGGCGGCTGTGACGCTCTGCCGCCTGC

GCAGCCCCCCCAAGAAAGGCCTGGGCTCCCCCACCGTGCACAAGATCTCCCGCTTCCCGCTCAAGCGACA

GGTGTCCCTGGAGTCCAACGCGTCCATGAGCTCCAACACACCACTGGTGCGCATCGCAAGGCTGTCCTCA

GGGGAGGGCCCCACGCTGGCCAATGTCTCCGAGCTCGAGCTGCCTGCCGACCCCAAATGGGAGCTGTCTC

GGGCCCGGCTGACCCTGGGCAAGCCCCTTGGGGAGGGCTGCTTCGGCCAGGTGGTCATGGCGGAGGCCAT

CGGCATTGACAAGGACCGGGCCGCCAAGCCTGTCACCGTAGCCGTGAAGATGCTGAAAGACGATGCCACT

GACAAGGACCTGTCGGACCTGGTGTCTGAGATGGAGATGATGAAGATGATCGGGAAACACAAAAACATCA

TCAACCTGCTGGGCGCCTGCACGCAGGGCGGGCCCCTGTACGTGCTGGTGGAGTACGCGGCCAAGGGTAA

CCTGCGGGAGTTTCTGCGGGCGCGGCGGCCCCCGGGCCTGGACTACTCCTTCGACACCTGCAAGCCGCCC

GAGGAGCAGCTCACCTTCAAGGACCTGGTGTCCTGTGCCTACCAGGTGGCCCGGGGCATGGAGTACTTGG

CCTCCCAGAAGTGCATCCACAGGGACCTGGCTGCCCGCAATGTGCTGGTGACCGAGGACAACGTGATGAA

GATCGCAGACTTCGGGCTGGCCCGGGACGTGCACAACCTCGACTACTACAAGAAGACGACCAACGGCCGG

CTGCCCGTGAAGTGGATGGCGCCTGAGGCCTTGTTTGACCGAGTCTACACTCACCAGAGTGACGTCTGGT

CCTTTGGGGTCCTGCTCTGGGAGATCTTCACGCTGGGGGGCTCCCCGTACCCCGGCATCCCTGTGGAGGA

GCTCTTCAAGCTGCTGAAGGAGGGCCACCGCATGGACAAGCCCGCCAACTGCACACACGACCTGTACATG

ATCATGCGGGAGTGCTGGCATGCCGCGCCCTCCCAGAGGCCCACCTTCAAGCAGCTGGTGGAGGACCTGG

ACCGTGTCCTTACCGTGACGTCCACCGACGAGTACCTGGACCTGTCGGCGCCTTTCGAGCAGTACTCCCC

GGGTGGCCAGGACACCCCCAGCTCCAGCTCCTCAGGGGACGACTCCGTGTTTGCCCACGACCTGCTGCCC

CCGGCCCCACCCAGCAGTGGGGGCTCGCGGACGTGAAGGGCCACTGGTCCCCAACAATGTGAGGGGTCCC

TAGCAGCCCACCCTGCTGCTGGTGCACAGCCACTCCCCGGCATGAGACTCAGTGCAGATGGAGAGACAGC

TACACAGAGCTTTGGTCTGTGTGTGTGTGTGTGCGTGTGTGTGTGTGTGTGTGCACATCCGCGTGTGCCT

GTGTGCGTGCGCATCTTGCCTCCAGGTGCAGAGGTACCCTGGGTGTCCCCGCTGCTGTGCAACGGTCTCC

TGACTGGTGCTGCAGCACCGAGGGGCCTTTGTTCTGGGGGGACCCAGTGCAGAATGTAAGTGGGCCCACC

CGGTGGGACCCCCGTGGGGCAGGGAGCTGGGCCCGACATGGCTCCGGCCTCTGCCTTTGCACCACGGGAC

ATCACAGGGTGGGCCTCGGCCCCTCCCACACCCAAAGCTGAGCCTGCAGGGAAGCCCCACATGTCCAGCA

CCTTGTGCCTGGGGTGTTAGTGGCACCGCCTCCCCACCTCCAGGCTTTCCCACTTCCCACCCTGCCCCTC

AGAGACTGAAATTACGGGTACCTGAAGATGGGAGCCTTTACCTTTTATGCAAAAGGTTTATTCCGGAAAC

TAGTGTACATTTCTATAAATAGATGCTGTGTATATGGTATATATACATATATATATATAACATATATGGA

AGAGGAAAAGGCTGGTACAACGGAGGCCTGCGACCCTGGGGGCACAGGAGGCAGGCATGGCCCTGGGCGG

GGCGTGGGGGGGCGTGGAGGGAGGCCCCAGGGGGTCTCACCCATGCAAGCAGAGGACCAGGGCCTTTTCT

GGCACCGCAGTTTTGTTTTAAAACTGGACCTGTATATTTGTAAAGCTATTTATGGGCCCCTGGCACTCTT

GTTCCCACACCCCAACACTTCCAGCATTTAGCTGGCCACATGGCGGAGAGTTTTAATTTTTAACTTATTG

ACAACCGAGAAGGTTTATCCCGCCGATAGAGGGACGGCCAAGAATGTACGTCCAGCCTGCCCCGGAGCTG

GAGGATCCCCTCCAAGCCTAAAAGGTTGTTAATAGTTGGAGGTGATTCCAGTGAAGATATTTTATTTCCT

TTGTCCTTTTTCAGGAGAATTAGATTTCTATAGGATTTTTCTTTAGGAGATTTATTTTTTGGACTTCAAA

GCAAGCTGGTATTTTCATACAAATTCTTCTAATTGCTGTGTGTCCCAGGCAGGGAGACGGTTTCCAGGGA

GGGGCCGGCCCTGTGTGCAGGTTCCGATGTTATTAGATGTTACAAGTTTATATATATCTATATATATAAT

TTATTGAGTTTTTACAAGATGTATTTGTTGTAGACTTAACACTTCTTACGCAATGCTTCTAGAGTTTTAT

AGCCTGGACTGCTACCTTTCAAAGCTTGGAGGGAAGCCGTGAATTCAGTTGGTTCGTTCTGTACTGTTAC

TGGGCCCTGAGTCTGGGCAGCTGTCCCTTGCTTGCCTGCAGGGCCATGGCTCAGGGTGGTCTCTTCTTGG

GGCCCAGTGCATGGTGGCCAGAGGTGTCACCCAAACCGGCAGGTGCGATTTTGTTAACCCAGCGACGAAC

TTTCCGAAAAATAAAGACACCTGGTTGCTAA

7. Motif

Pfam (12 motifs)

Pfam	Position(Independent E-value)	Description
PK_Tyr_Ser-Thr	472..748(6e-104)	PF07714, Protein tyrosine and serine/threonine kinase
Pkinase	475..746(6.1e-44)	PF00069, Protein kinase domain
I-set	48..110(0.009) 166..245(3.7e-15) 260..356(2.8e-10)	PF07679, Immunoglobulin I-set domain
Ig_3	51..110(0.0014) 166..232(2.6e-12) 260..343(6.3e-11)	PF13927, Immunoglobulin domain
Ig_2	52..115(0.0049) 166..240(8.9e-10) 253..347(0.00011)	PF13895, Immunoglobulin domain
ig	49..110(0.082) 161..236(5.3e-08) 264..347(1.8e-05)	PF00047, Immunoglobulin domain
V-set	201..245(0.14) 260..357(6.4e-05)	PF07686, Immunoglobulin V-set domain
C2-set_2	170..195(0.05)	PF08205, CD80-like C2-set immunoglobulin domain
ABC1	588..643(0.12)	PF03109, ABC1 atypical kinase-like domain
Pkinase_fungal	589..680(0.03)	PF17667, Fungal protein kinase
TCAD9	611..641(0.3)	PF19974, Ternary complex associated domain 9
DUF1533	288..338(0.5)	PF07550, Protein of unknown function (DUF1533)

8. Evolutionary Relationship

Evolutionary analysis by Maximum Likelihood method

The evolutionary history was inferred by using the Maximum Likelihood method and JTT matrix-based model [1]. The tree with the highest log likelihood (-4663.32) is shown. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using a JTT model, and then selecting the topology with superior log likelihood value. The proportion of sites where at least 1 unambiguous base is present in at least 1 sequence for each descendent clade is shown next to each internal node in the tree. This analysis involved 100 amino acid sequences. There were a total of 826 positions in the final dataset

9. SNP

SNP	COORDINATE	AMINO ACID CHANGE	GENE ID	TRANSCRIPT ID	PROTEIN ID	SIFT SCORE	SIFT MEDIAN	SIFT PREDICTION
rs4647924	1803571	P70R	ENSG00000068078	ENST00000507588	ENSP00000427289	0.001	3.83	DELETERIOUS (WARNING LOW CONFIDENCE)
rs4647924	1803571	P250R	ENSG00000068078	ENST00000481110	ENSP00000420533	0.144	2.57	TOLERATED
rs4647924	1803571	P250R	ENSG00000068078	ENST00000340107	ENSP00000339824	0.191	2.36	TOLERATED
rs4647924	1803571	P250R	ENSG00000068078	ENST00000352904	ENSP00000231803	0.195	2.4	TOLERATED
rs4647924	1803571	P250R	ENSG00000068078	ENST00000412135	ENSP00000412903	0.195	2.4	TOLERATED
rs4647924	1803571	P250R	ENSG00000068078	ENST00000260795	ENSP00000260795	0.225	2.36	TOLERATED
rs4647924	1803571	P250R	ENSG00000068078	ENST00000440486	ENSP00000414914	0.225	2.36	TOLERATED
rs28928868	1807891	K652N	ENSG00000068078	ENST00000340107	ENSP00000339824	0	2.37	DELETERIOUS
rs28928868	1807891	K650N	ENSG00000068078	ENST00000260795	ENSP00000260795	0.001	2.35	DELETERIOUS
rs28928868	1807891	K538N	ENSG00000068078	ENST00000352904	ENSP00000231803	0.001	2.37	DELETERIOUS
rs28928868	1807891	K538N	ENSG00000068078	ENST00000412135	ENSP00000412903	0.001	2.37	DELETERIOUS
rs28928868	1807891	K650N	ENSG00000068078	ENST00000440486	ENSP00000414914	0.001	2.35	DELETERIOUS
rs28928868	1807891	K651N	ENSG00000068078	ENST00000481110	ENSP00000420533	0.001	2.54	DELETERIOUS
rs28928868	1807891	K652N	ENSG00000068078	ENST00000340107	ENSP00000339824	0	2.37	DELETERIOUS
rs28928868	1807891	K650N	ENSG00000068078	ENST00000260795	ENSP00000260795	0.001	2.35	DELETERIOUS
rs28928868	1807891	K538N	ENSG00000068078	ENST00000352904	ENSP00000231803	0.001	2.37	DELETERIOUS
rs28928868	1807891	K538N	ENSG00000068078	ENST00000412135	ENSP00000412903	0.001	2.37	DELETERIOUS
rs28928868	1807891	K650N	ENSG00000068078	ENST00000440486	ENSP00000414914	0.001	2.35	DELETERIOUS
rs28928868	1807891	K651N	ENSG00000068078	ENST00000481110	ENSP00000420533	0.001	2.54	DELETERIOUS
rs28931614	1806119	G380R	ENSG00000068078	ENST00000260795	ENSP00000260795	0.139	2.39	TOLERATED
rs28931614	1806119	G382R	ENSG00000068078	ENST00000340107	ENSP00000339824	0.139	2.4	TOLERATED
rs28931614	1806119	G380R	ENSG00000068078	ENST00000440486	ENSP00000414914	0.139	2.39	TOLERATED
rs28931614	1806119	G380R	ENSG00000068078	ENST00000481110	ENSP00000420533	0.239	2.59	TOLERATED
rs28931614	1806119	G380R	ENSG00000068078	ENST00000260795	ENSP00000260795	0.139	2.39	TOLERATED
rs28931614	1806119	G382R	ENSG00000068078	ENST00000340107	ENSP00000339824	0.139	2.4	TOLERATED
rs28931614	1806119	G380R	ENSG00000068078	ENST00000440486	ENSP00000414914	0.139	2.39	TOLERATED
rs28931614	1806119	G380R	ENSG00000068078	ENST00000481110	ENSP00000420533	0.239	2.59	TOLERATED
rs28931615	1806153	A391E	ENSG00000068078	ENST00000260795	ENSP00000260795	0.073	2.36	TOLERATED
rs28931615	1806153	A391E	ENSG00000068078	ENST00000440486	ENSP00000414914	0.073	2.36	TOLERATED
rs28931615	1806153	A393E	ENSG00000068078	ENST00000340107	ENSP00000339824	0.08	2.36	TOLERATED
rs28931615	1806153	A391E	ENSG00000068078	ENST00000481110	ENSP00000420533	0.112	2.54	TOLERATED
rs28933068	1807371	N541K	ENSG00000068078	ENST00000481110	ENSP00000420533	0.001	2.52	DELETERIOUS
rs28933068	1807371	N540K	ENSG00000068078	ENST00000260795	ENSP00000260795	0.002	2.35	DELETERIOUS
rs28933068	1807371	N542K	ENSG00000068078	ENST00000340107	ENSP00000339824	0.002	2.37	DELETERIOUS
rs28933068	1807371	N540K	ENSG00000068078	ENST00000440486	ENSP00000414914	0.002	2.35	DELETERIOUS
rs28933068	1807371	N428K	ENSG00000068078	ENST00000352904	ENSP00000231803	0.028	2.37	DELETERIOUS
rs28933068	1807371	N428K	ENSG00000068078	ENST00000412135	ENSP00000412903	0.028	2.37	DELETERIOUS
rs28933068	1807371	N541K	ENSG00000068078	ENST00000481110	ENSP00000420533	0.001	2.52	DELETERIOUS
rs28933068	1807371	N540K	ENSG00000068078	ENST00000260795	ENSP00000260795	0.002	2.35	DELETERIOUS
rs28933068	1807371	N542K	ENSG00000068078	ENST00000340107	ENSP00000339824	0.002	2.37	DELETERIOUS
rs28933068	1807371	N540K	ENSG00000068078	ENST00000440486	ENSP00000414914	0.002	2.35	DELETERIOUS
rs28933068	1807371	N428K	ENSG00000068078	ENST00000352904	ENSP00000231803	0.028	2.37	DELETERIOUS
rs28933068	1807371	N428K	ENSG00000068078	ENST00000412135	ENSP00000412903	0.028	2.37	DELETERIOUS
rs75790268	1806104	G375C	ENSG00000068078	ENST00000260795	ENSP00000260795	0.043	2.37	DELETERIOUS
rs75790268	1806104	G375C	ENSG00000068078	ENST00000440486	ENSP00000414914	0.043	2.37	DELETERIOUS
rs75790268	1806104	G377C	ENSG00000068078	ENST00000340107	ENSP00000339824	0.046	2.39	DELETERIOUS
rs75790268	1806104	G375C	ENSG00000068078	ENST00000481110	ENSP00000420533	0.049	2.57	DELETERIOUS
rs77722678	1807370	N540T	ENSG00000068078	ENST00000260795	ENSP00000260795	0.002	2.35	DELETERIOUS
rs77722678	1807370	N542T	ENSG00000068078	ENST00000340107	ENSP00000339824	0.002	2.37	DELETERIOUS
rs77722678	1807370	N540T	ENSG00000068078	ENST00000440486	ENSP00000414914	0.002	2.35	DELETERIOUS
rs77722678	1807370	N541T	ENSG00000068078	ENST00000481110	ENSP00000420533	0.013	2.52	DELETERIOUS
rs77722678	1807370	N428T	ENSG00000068078	ENST00000352904	ENSP00000231803	0.029	2.37	DELETERIOUS
rs77722678	1807370	N428T	ENSG00000068078	ENST00000412135	ENSP00000412903	0.029	2.37	DELETERIOUS
rs77722678	1807370	N428S	ENSG00000068078	ENST00000352904	ENSP00000231803	0.02	2.37	DELETERIOUS
rs77722678	1807370	N428S	ENSG00000068078	ENST00000412135	ENSP00000412903	0.02	2.37	DELETERIOUS
rs77722678	1807370	N540S	ENSG00000068078	ENST00000260795	ENSP00000260795	0.026	2.35	DELETERIOUS
rs77722678	1807370	N540S	ENSG00000068078	ENST00000440486	ENSP00000414914	0.026	2.35	DELETERIOUS
rs77722678	1807370	N542S	ENSG00000068078	ENST00000340107	ENSP00000339824	0.027	2.37	DELETERIOUS
rs77722678	1807370	N541S	ENSG00000068078	ENST00000481110	ENSP00000420533	0.05	2.52	TOLERATED
rs78311289	1807889	K652Q	ENSG00000068078	ENST00000340107	ENSP00000339824	0	2.37	DELETERIOUS
rs78311289	1807889	K651Q	ENSG00000068078	ENST00000481110	ENSP00000420533	0.001	2.54	DELETERIOUS
rs78311289	1807889	K650Q	ENSG00000068078	ENST00000260795	ENSP00000260795	0.002	2.35	DELETERIOUS
rs78311289	1807889	K538Q	ENSG00000068078	ENST00000352904	ENSP00000231803	0.002	2.37	DELETERIOUS
rs78311289	1807889	K538Q	ENSG00000068078	ENST00000412135	ENSP00000412903	0.002	2.37	DELETERIOUS
rs78311289	1807889	K650Q	ENSG00000068078	ENST00000440486	ENSP00000414914	0.002	2.35	DELETERIOUS
rs78311289	1807889	K652E	ENSG00000068078	ENST00000340107	ENSP00000339824	0	2.37	DELETERIOUS
rs78311289	1807889	K650E	ENSG00000068078	ENST00000260795	ENSP00000260795	0.001	2.35	DELETERIOUS
rs78311289	1807889	K538E	ENSG00000068078	ENST00000352904	ENSP00000231803	0.001	2.37	DELETERIOUS
rs78311289	1807889	K538E	ENSG00000068078	ENST00000412135	ENSP00000412903	0.001	2.37	DELETERIOUS
rs78311289	1807889	K650E	ENSG00000068078	ENST00000440486	ENSP00000414914	0.001	2.35	DELETERIOUS
rs78311289	1807889	K651E	ENSG00000068078	ENST00000481110	ENSP00000420533	0.001	2.54	DELETERIOUS
rs80053154	1807363	I538V	ENSG00000068078	ENST00000260795	ENSP00000260795	0.032	2.35	DELETERIOUS
rs80053154	1807363	I538V	ENSG00000068078	ENST00000440486	ENSP00000414914	0.032	2.35	DELETERIOUS
rs80053154	1807363	I540V	ENSG00000068078	ENST00000340107	ENSP00000339824	0.033	2.37	DELETERIOUS
rs80053154	1807363	I426V	ENSG00000068078	ENST00000352904	ENSP00000231803	0.068	2.37	TOLERATED
rs80053154	1807363	I426V	ENSG00000068078	ENST00000412135	ENSP00000412903	0.068	2.37	TOLERATED
rs80053154	1807363	I539V	ENSG00000068078	ENST00000481110	ENSP00000420533	0.135	2.52	TOLERATED
rs121913101	1808987	*807R	ENSG00000068078	ENST00000260795	ENSP00000260795
rs121913101	1808987	*809R	ENSG00000068078	ENST00000340107	ENSP00000339824
rs121913101	1808987	*695R	ENSG00000068078	ENST00000352904	ENSP00000231803
rs121913101	1808987	*695R	ENSG00000068078	ENST00000412135	ENSP00000412903
rs121913101	1808987	*807R	ENSG00000068078	ENST00000440486	ENSP00000414914
rs121913101	1808987	V784E	ENSG00000068078	ENST00000481110	ENSP00000420533
rs121913101	1808987	*807G	ENSG00000068078	ENST00000260795	ENSP00000260795
rs121913101	1808987	*809G	ENSG00000068078	ENST00000340107	ENSP00000339824
rs121913101	1808987	*695G	ENSG00000068078	ENST00000352904	ENSP00000231803
rs121913101	1808987	*695G	ENSG00000068078	ENST00000412135	ENSP00000412903
rs121913101	1808987	*807G	ENSG00000068078	ENST00000440486	ENSP00000414914
rs121913101	1808987	V784G	ENSG00000068078	ENST00000481110	ENSP00000420533
rs121913103	1808989	K785*	ENSG00000068078	ENST00000481110	ENSP00000420533
rs121913103	1808989	*807C	ENSG00000068078	ENST00000260795	ENSP00000260795
rs121913103	1808989	*809C	ENSG00000068078	ENST00000340107	ENSP00000339824
rs121913103	1808989	*695C	ENSG00000068078	ENST00000352904	ENSP00000231803
rs121913103	1808989	*695C	ENSG00000068078	ENST00000412135	ENSP00000412903
rs121913103	1808989	*807C	ENSG00000068078	ENST00000440486	ENSP00000414914
rs121913105	1807890	K650M	ENSG00000068078	ENST00000260795	ENSP00000260795	0	2.35	DELETERIOUS
rs121913105	1807890	K652M	ENSG00000068078	ENST00000340107	ENSP00000339824	0	2.37	DELETERIOUS
rs121913105	1807890	K538M	ENSG00000068078	ENST00000352904	ENSP00000231803	0	2.37	DELETERIOUS
rs121913105	1807890	K538M	ENSG00000068078	ENST00000412135	ENSP00000412903	0	2.37	DELETERIOUS
rs121913105	1807890	K650M	ENSG00000068078	ENST00000440486	ENSP00000414914	0	2.35	DELETERIOUS
rs121913105	1807890	K651M	ENSG00000068078	ENST00000481110	ENSP00000420533	0	2.54	DELETERIOUS
rs121913111	1805452	E108K	ENSG00000068078	ENST00000507588	ENSP00000427289	0	3.88	DELETERIOUS (WARNING LOW CONFIDENCE)
rs121913111	1805452	E322K	ENSG00000068078	ENST00000481110	ENSP00000420533	0.032	2.6	DELETERIOUS
rs121913111	1805452	E322K	ENSG00000068078	ENST00000260795	ENSP00000260795	0.037	2.38	DELETERIOUS
rs121913111	1805452	E322K	ENSG00000068078	ENST00000440486	ENSP00000414914	0.037	2.38	DELETERIOUS
rs121913112	1807288	D514N	ENSG00000068078	ENST00000481110	ENSP00000420533	0.285	2.54	TOLERATED
rs121913112	1807288	D515N	ENSG00000068078	ENST00000340107	ENSP00000339824	0.344	2.36	TOLERATED
rs121913112	1807288	D513N	ENSG00000068078	ENST00000260795	ENSP00000260795	0.348	2.36	TOLERATED
rs121913112	1807288	D513N	ENSG00000068078	ENST00000440486	ENSP00000414914	0.348	2.36	TOLERATED
rs121913112	1807288	D401N	ENSG00000068078	ENST00000352904	ENSP00000231803	0.375	2.37	TOLERATED
rs121913112	1807288	D401N	ENSG00000068078	ENST00000412135	ENSP00000412903	0.375	2.37	TOLERATED
rs121913113	1807803	R621H	ENSG00000068078	ENST00000260795	ENSP00000260795	0	2.35	DELETERIOUS
rs121913113	1807803	R623H	ENSG00000068078	ENST00000340107	ENSP00000339824	0	2.37	DELETERIOUS
rs121913113	1807803	R509H	ENSG00000068078	ENST00000352904	ENSP00000231803	0	2.37	DELETERIOUS
rs121913113	1807803	R509H	ENSG00000068078	ENST00000412135	ENSP00000412903	0	2.37	DELETERIOUS
rs121913113	1807803	R621H	ENSG00000068078	ENST00000440486	ENSP00000414914	0	2.35	DELETERIOUS
rs121913113	1807803	R622H	ENSG00000068078	ENST00000481110	ENSP00000420533	0	2.52	DELETERIOUS
rs121913114	1803657	S279C	ENSG00000068078	ENST00000260795	ENSP00000260795	0.001	2.36	DELETERIOUS
rs121913114	1803657	S279C	ENSG00000068078	ENST00000340107	ENSP00000339824	0.001	2.36	DELETERIOUS
rs121913114	1803657	S279C	ENSG00000068078	ENST00000352904	ENSP00000231803	0.001	2.4	DELETERIOUS
rs121913114	1803657	S279C	ENSG00000068078	ENST00000412135	ENSP00000412903	0.001	2.4	DELETERIOUS
rs121913114	1803657	S279C	ENSG00000068078	ENST00000440486	ENSP00000414914	0.001	2.36	DELETERIOUS
rs121913114	1803657	S279C	ENSG00000068078	ENST00000481110	ENSP00000420533	0.001	2.56	DELETERIOUS
rs121913115	1803655	Y278C	ENSG00000068078	ENST00000340107	ENSP00000339824	0.006	2.36	DELETERIOUS
rs121913115	1803655	Y278C	ENSG00000068078	ENST00000260795	ENSP00000260795	0.014	2.36	DELETERIOUS
rs121913115	1803655	Y278C	ENSG00000068078	ENST00000440486	ENSP00000414914	0.014	2.36	DELETERIOUS
rs121913115	1803655	Y278C	ENSG00000068078	ENST00000481110	ENSP00000420533	0.016	2.56	DELETERIOUS
rs121913115	1803655	Y278C	ENSG00000068078	ENST00000352904	ENSP00000231803	0.019	2.4	DELETERIOUS
rs121913115	1803655	Y278C	ENSG00000068078	ENST00000412135	ENSP00000412903	0.019	2.4	DELETERIOUS
rs121913116	1801122	S84L	ENSG00000068078	ENST00000481110	ENSP00000420533	0.051	2.91	TOLERATED
rs121913116	1801122	S84L	ENSG00000068078	ENST00000352904	ENSP00000231803	0.108	2.6	TOLERATED
rs121913116	1801122	S84L	ENSG00000068078	ENST00000412135	ENSP00000412903	0.108	2.6	TOLERATED
rs121913116	1801122	S84L	ENSG00000068078	ENST00000260795	ENSP00000260795	0.146	2.51	TOLERATED
rs121913116	1801122	S84L	ENSG00000068078	ENST00000340107	ENSP00000339824	0.146	2.52	TOLERATED
rs121913116	1801122	S84L	ENSG00000068078	ENST00000440486	ENSP00000414914	0.146	2.51	TOLERATED
rs121913479	1806089	G370C	ENSG00000068078	ENST00000481110	ENSP00000420533	0.077	2.55	TOLERATED
rs121913479	1806089	G370C	ENSG00000068078	ENST00000260795	ENSP00000260795	0.152	2.38	TOLERATED
rs121913479	1806089	G370C	ENSG00000068078	ENST00000440486	ENSP00000414914	0.152	2.38	TOLERATED
rs121913479	1806089	G372C	ENSG00000068078	ENST00000340107	ENSP00000339824	0.173	2.36	TOLERATED
rs121913482	1803564	R248C	ENSG00000068078	ENST00000260795	ENSP00000260795	0	2.36	DELETERIOUS
rs121913482	1803564	R248C	ENSG00000068078	ENST00000340107	ENSP00000339824	0	2.36	DELETERIOUS
rs121913482	1803564	R248C	ENSG00000068078	ENST00000352904	ENSP00000231803	0	2.41	DELETERIOUS
rs121913482	1803564	R248C	ENSG00000068078	ENST00000412135	ENSP00000412903	0	2.41	DELETERIOUS
rs121913482	1803564	R248C	ENSG00000068078	ENST00000440486	ENSP00000414914	0	2.36	DELETERIOUS
rs121913482	1803564	R248C	ENSG00000068078	ENST00000481110	ENSP00000420533	0	2.57	DELETERIOUS
rs121913482	1803564	R68C	ENSG00000068078	ENST00000507588	ENSP00000427289	0	3.83	DELETERIOUS (WARNING LOW CONFIDENCE)
rs121913483	1803568	S69C	ENSG00000068078	ENST00000507588	ENSP00000427289	0	3.83	DELETERIOUS (WARNING LOW CONFIDENCE)
rs121913483	1803568	S249C	ENSG00000068078	ENST00000481110	ENSP00000420533	0.103	2.57	TOLERATED
rs121913483	1803568	S249C	ENSG00000068078	ENST00000352904	ENSP00000231803	0.172	2.41	TOLERATED
rs121913483	1803568	S249C	ENSG00000068078	ENST00000412135	ENSP00000412903	0.172	2.41	TOLERATED
rs121913483	1803568	S249C	ENSG00000068078	ENST00000260795	ENSP00000260795	0.173	2.36	TOLERATED
rs121913483	1803568	S249C	ENSG00000068078	ENST00000440486	ENSP00000414914	0.173	2.36	TOLERATED
rs121913483	1803568	S249C	ENSG00000068078	ENST00000340107	ENSP00000339824	0.174	2.36	TOLERATED
rs121913484	1806092	S373C	ENSG00000068078	ENST00000340107	ENSP00000339824	0.052	2.31	TOLERATED
rs121913484	1806092	S371C	ENSG00000068078	ENST00000260795	ENSP00000260795	0.065	2.33	TOLERATED
rs121913484	1806092	S371C	ENSG00000068078	ENST00000440486	ENSP00000414914	0.065	2.33	TOLERATED
rs121913484	1806092	S371C	ENSG00000068078	ENST00000481110	ENSP00000420533	0.185	2.54	TOLERATED
rs121913485	1806099	Y375C	ENSG00000068078	ENST00000340107	ENSP00000339824	0.029	2.35	DELETERIOUS
rs121913485	1806099	Y373C	ENSG00000068078	ENST00000481110	ENSP00000420533	0.035	2.54	DELETERIOUS
rs121913485	1806099	Y373C	ENSG00000068078	ENST00000260795	ENSP00000260795	0.039	2.35	DELETERIOUS
rs121913485	1806099	Y373C	ENSG00000068078	ENST00000440486	ENSP00000414914	0.039	2.35	DELETERIOUS
rs267606808	1807123	Q487R	ENSG00000068078	ENST00000340107	ENSP00000339824	0.209	2.37	TOLERATED
rs267606808	1807123	Q485R	ENSG00000068078	ENST00000260795	ENSP00000260795	0.241	2.36	TOLERATED
rs267606808	1807123	Q485R	ENSG00000068078	ENST00000440486	ENSP00000414914	0.241	2.36	TOLERATED
rs267606808	1807123	Q486R	ENSG00000068078	ENST00000481110	ENSP00000420533	0.241	2.54	TOLERATED
rs267606808	1807123	Q373R	ENSG00000068078	ENST00000352904	ENSP00000231803	0.367	2.37	TOLERATED
rs267606808	1807123	Q373R	ENSG00000068078	ENST00000412135	ENSP00000412903	0.367	2.37	TOLERATED
rs267606809	1806111	L379R	ENSG00000068078	ENST00000340107	ENSP00000339824	0.083	2.35	TOLERATED
rs267606809	1806111	L377R	ENSG00000068078	ENST00000481110	ENSP00000420533	0.09	2.55	TOLERATED
rs267606809	1806111	L377R	ENSG00000068078	ENST00000260795	ENSP00000260795	0.129	2.35	TOLERATED
rs267606809	1806111	L377R	ENSG00000068078	ENST00000440486	ENSP00000414914	0.129	2.35	TOLERATED

Evolutionary analysis of coding SNPs

SNP	substitution	Message	Pdel
rs4647924	P250R	probably damaging	0.74
rs4647924	P250R	probably damaging	0.74
rs4647924	P250R	probably damaging	0.74
rs4647924	P250R	probably damaging	0.74
rs4647924	P250R	probably damaging	0.74
rs4647924	P250R	probably damaging	0.74
rs28928868	K650N	probably damaging	0.85
rs28928868	K650N	probably damaging	0.85
rs28928868	K650N	probably damaging	0.85
rs28928868	K650N	probably damaging	0.85
rs28931614	G380R	possibly damaging	0.5
rs28931614	G382R	probably damaging	0.74
rs28931614	G380R	possibly damaging	0.5
rs28931614	G380R	possibly damaging	0.5
rs28931614	G380R	possibly damaging	0.5
rs28931614	G382R	probably damaging	0.74
rs28931614	G380R	possibly damaging	0.5
rs28931614	G380R	possibly damaging	0.5
rs28931615	A391E	probably benign	0.27
rs28931615	A391E	probably benign	0.27
rs28931615	A391E	probably benign	0.27
rs28933068	N540K	probably damaging	0.85
rs28933068	N540K	probably damaging	0.85
rs28933068	N428K	probably damaging	0.57
rs28933068	N428K	probably damaging	0.57
rs28933068	N540K	probably damaging	0.85
rs28933068	N540K	probably damaging	0.85
rs28933068	N428K	probably damaging	0.57
rs28933068	N428K	probably damaging	0.57
rs75790268	G375C	possibly damaging	0.5
rs75790268	G375C	possibly damaging	0.5
rs75790268	G375C	possibly damaging	0.5
rs77722678	N540T	probably damaging	0.85
rs77722678	N540T	probably damaging	0.85
rs77722678	N428T	probably damaging	0.57
rs77722678	N428T	probably damaging	0.57
rs77722678	N428S	probably damaging	0.57
rs77722678	N428S	probably damaging	0.57
rs77722678	N540S	probably damaging	0.85
rs77722678	N540S	probably damaging	0.85
rs78311289	K650Q	probably damaging	0.85
rs78311289	K650Q	probably damaging	0.85
rs78311289	K650E	probably damaging	0.85
rs78311289	K650E	probably damaging	0.85
rs80053154	I538V	probably damaging	0.85
rs80053154	I538V	probably damaging	0.85
rs80053154	I539V	probably damaging	0.85
rs121913101	K650M	probably damaging	0.85
rs121913101	K650M	probably damaging	0.85
rs121913101	E322K	probably damaging	0.74
rs121913101	E322K	probably damaging	0.74
rs121913101	E322K	probably damaging	0.74
rs121913103	D513N	probably damaging	0.78
rs121913103	D513N	probably damaging	0.78
rs121913105	R621H	probably damaging	0.85
rs121913105	R621H	probably damaging	0.85
rs121913111	S279C	probably damaging	0.74
rs121913111	S279C	probably damaging	0.74
rs121913111	S279C	probably damaging	0.74
rs121913111	S279C	probably damaging	0.74
rs121913112	S279C	probably damaging	0.74
rs121913112	S279C	probably damaging	0.74
rs121913112	Y278C	probably damaging	0.74
rs121913112	Y278C	probably damaging	0.74
rs121913112	Y278C	probably damaging	0.74
rs121913112	Y278C	probably damaging	0.74
rs121913113	Y278C	probably damaging	0.74
rs121913113	Y278C	probably damaging	0.74
rs121913113	S84L	probably benign	0.27
rs121913113	S84L	probably benign	0.27
rs121913113	S84L	probably benign	0.27
rs121913113	S84L	probably benign	0.27
rs121913114	S84L	probably benign	0.27
rs121913114	S84L	probably benign	0.27
rs121913114	G370C	possibly damaging	0.5
rs121913114	G370C	possibly damaging	0.5
rs121913114	G370C	possibly damaging	0.5
rs121913115	R248C	probably damaging	0.74
rs121913115	R248C	probably damaging	0.74
rs121913115	R248C	probably damaging	0.74
rs121913115	R248C	probably damaging	0.74
rs121913115	R248C	probably damaging	0.74
rs121913115	R248C	probably damaging	0.74
rs121913116	S249C	probably damaging	0.74
rs121913116	S249C	probably damaging	0.74
rs121913116	S249C	probably damaging	0.74
rs121913116	S249C	probably damaging	0.74
rs121913479	S249C	probably damaging	0.74
rs121913479	S249C	probably damaging	0.74
rs121913479	S371C	possibly damaging	0.5
rs121913482	S371C	possibly damaging	0.5
rs121913482	S371C	possibly damaging	0.5
rs121913482	Y373C	probably damaging	0.74
rs121913482	Y373C	probably damaging	0.74
rs121913482	Y373C	probably damaging	0.74
rs121913483	Q485R	probably damaging	0.74
rs121913483	Q485R	probably damaging	0.74
rs121913483	L377R	possibly damaging	0.5
rs121913484	L377R	possibly damaging	0.5
rs121913484	L377R	possibly damaging	0.5

Provean

SNP IDS	AMINO ACID CHANGE	Probability	Prediction	Score
rs121918550	S263P	Damaging	Deleterious	-4.12
rs121918550	S230P	Damaging	Deleterious	-4.12
rs121918550	L139P	Damaging	Deleterious	-4.12
rs121918551	L397F	Damaging	Neutral	-2.27
rs121918551	L430F	Damaging	Neutral	-2.27
rs121918551	D306F	Tolarated	Neutral	-2.27

SNAP 2

Wildtype Amino Acid	Position	Variant Amino Acid	Predicted Effect	Score	Expected Accuracy
S	263	P	effect	85	91%
S	230	P	effect	73	85%
L	139	p	neutral	-29	61%
L	397	F	effect	25	63%
L	430	F	effect	2	53%
D	306	F	effect	75	85%

Polyphen 2

Rs_Id	Mutation Probability	Score
rs4647924	Possibly damaging	1.00
rs4647924	Probably damaging	0.999
rs4647924	Probably damaging	0.98
rs4647924	Possibly damaging	1.00
rs4647924	Possibly damaging	1.00
rs4647924	Probably damaging	0.999
rs28928868	Probably damaging	0.98
rs28928868	Probably damaging	0.999
rs28928868	Possibly damaging	1.00
rs28928868	Probably damaging	0.999
rs28931614	Probably damaging	0.98
rs28931614	Probably damaging	0.999
rs28931614	Probably damaging	0.98
rs28931614	Possibly damaging	0.98
rs28931614	Possibly damaging	1.00
rs28931614	Probably damaging	0.999
rs28931614	Probably damaging	0.98
rs28931614	Possibly damaging	1.00
rs28931615	Possibly damaging	1.00
rs28931615	Probably damaging	0.999
rs28931615	Probably damaging	0.98
rs28933068	Possibly damaging	1.00
rs28933068	Probably damaging	0.98
rs28933068	Possibly damaging	1.00
rs28933068	Probably damaging	0.999
rs28933068	Probably damaging	0.98
rs28933068	Possibly damaging	1.00
rs28933068	Possibly damaging	1.00
rs28933068	Probably damaging	0.999
rs75790268	Probably damaging	0.98
rs75790268	Possibly damaging	1.00
rs75790268	Possibly damaging	1.00
rs77722678	Probably damaging	0.999
rs77722678	Possibly damaging	1.00
rs77722678	Probably damaging	0.999
rs77722678	Probably damaging	0.98
rs77722678	Possibly damaging	1.00
rs77722678	Possibly damaging	1.00
rs77722678	Possibly damaging	1.00
rs77722678	Probably damaging	0.999
rs78311289	Probably damaging	0.98
rs78311289	Probably damaging	0.98
rs78311289	Possibly damaging	1.00
rs78311289	Probably damaging	0.999
rs80053154	Probably damaging	0.98
rs80053154	Possibly damaging	1.00

Hope Prediction

Rs_ids	Mutation	Mapping issue	AA variant	Function. Impact	Score
rs121918550	AAAS_HUMAN S263P		S263P	high	4.145
rs121918550	AAAS_HUMAN S230P	Uniprot residue: R
rs121918550	AAAS_HUMAN L139P		L139P	medium	2.085
rs121918551	AAAS_HUMAN L397F	Uniprot residue: G
rs121918551	AAAS_HUMAN L430F		L430F	low	1.625
rs121918551	AAAS_HUMAN L306F	Uniprot residue: T

10. Active Site

Rank	C-Score	Cluster Size	PDB Hit	Lig Name	Consensus Building Residues
1	0.45	306	3gqlA	GQL	478,479,480,486,506,508,525,539, 555,556,557,558,624,635
2	0.27	194	5ew8A	5SF	478,486,506,508,525,529,539,553, 555,556,557,558,561,621,622,624, 634,635,636
3	0.04	20	1ad5A	CA	622,635
4	0.01	6	3lw0C	CCX	525,526,529,539,553,555,615,616, 633,634,655,656,671
5	0.01	13	2c0tB	CA	508,622,635
6	0.01	6	3bu3A	PEPTIDE	480,486,558,562,564,565,617,621, 650,651,652,653,654,655,656,657, 658,660,665,666,667,668,669,671, 693,700
7	0.01	8	1k3aA	PEPTIDE	617,621,650,652,653,654,655,656, 657,660,665,666,668,669,700
8	0.01	6	3qrkA	9DP	506,507,508,521,525,529,532,539, 553,555,634,635
9	0.00	1	3d94A	CA	518,641,643
10	0.00	1	3bprC	CL	695,696,713,714

11. Primary Sequence Analysis

Number of amino acids

Molecular weight

87709.64

Theoretical pI

5.59

Amino acid composition

Ala (A) 66 8.2%

Arg (R) 44 5.5%

Asn (N) 22 2.7%

Asp (D) 47 5.8%

Cys (C) 17 2.1%

Gln (Q) 26 3.2%

Glu (E) 53 6.6%

Gly (G) 68 8.4%

His (H) 20 2.5%

Ile (I) 22 2.7%

Leu (L) 80 9.9%

Lys (K) 37 4.6%

Met (M) 17 2.1%

Phe (F) 24 3.0%

Pro (P) 56 6.9%

Ser (S) 61 7.6%

Thr (T) 43 5.3%

Trp (W) 11 1.4%

Tyr (Y) 21 2.6%

Val (V) 71 8.8%

Pyl (O) 0 0.0%

Sec (U)   0              0.0%

Total number of negatively charged residues (Asp + Glu)

Total number of positively charged residues (Arg + Lys)

Formula

C₃₈₇₅H₆₁₀₆N₁₀₇₄O₁₁₈₀S₃₄

Total number of atoms(12269)

Carbon      C                3875

Hydrogen    H              6106

Nitrogen    N                1074

Oxygen      O               1180

Sulfur      S                     34

Instability index(II)

44.33

Stability

Protein is unstable

Aliphatic index(AI)

83.09

GRAVY

-0.242

12. Secondary structure analysis

Alpha helix (Hh) : 198 is 24.57%

310 helix (Gg) : 0 is 0.00%

Pi helix (Ii) : 0 is 0.00%

Beta bridge (Bb) : 0 is 0.00%

Extended strand (Ee) : 176 is 21.84%

Beta turn (Tt) : 37 is 4.59%

Bend region (Ss) : 0 is 0.00%

Random coil (Cc) : 395 is 49.01%

Ambiguous states (?) : 0 is 0.00%

Other states : 0 is 0.00

Secondary Structure Prediction

Amino Acid Types

13. Enzymes

Name of enzyme	No. of cleavages
Arg-C proteinase	44
Asp-N endopeptidase	47
Asp-N endopeptidase + N-terminal Glu	100
BNPS-Skatole	11
CNBr	17
Caspase1	1
Chymotrypsin-high specificity (C-term to [FYW], not before P)	53
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	163
Clostripain	44
Formic acid	47
Glutamyl endopeptidase	53
Hydroxylamine	3
Iodosobenzoic acid	11
LysC	37
LysN	37
NTCB (2-nitro-5-thiocyanobenzoic acid)	17
Pepsin (pH1.3)	150
Pepsin (pH>2)	190
Proline-endopeptidase	6
Proteinase K	391
Staphylococcal peptidase I	49
Thermolysin	225
Trypsin	71

14. Epitope

Peptide Rank	Start Position	Sequence	Score	Prediction
1	387	EDAGEYTCL	1	Epitope
2	506	LANVSELEL	1	Epitope
3	522	LSRARLTLG	1	Epitope
4	547	IGIDKDRAA	1	Epitope
5	696	DVHNLDYYK	1	Epitope

15. Protein-Protein Interaction

No of Nodes	11
No of Edges	32
Avg node degree	5.82
avg. local clustering coefficient	0.878
expected number of edges	11
p-value	1.81e-07
Protein – Protein Interaction network

16. MRNA