IGDRPT (Indian Genetic Disease Risk Prediction Tool)

Disease Name: Glycogen storage disease Ia

1 .Inheritance

Autosomal Recessive

2. Gene Name

G6PC

3. Protein Name

Glucose-6-phosphatase

4. Chromosomal Location

17q21

5. Protein Sequence

>sp|P35575|G6PC1_HUMAN Glucose-6-phosphatase catalytic subunit 1 OS=Homo sapiens OX=9606 GN=G6PC1 PE=1 SV=2

MEEGMNVLHDFGIQSTHYLQVNYQDSQDWFILVSVIADLRNAFYVLFPIWFHLQEAVGIK

LLWVAVIGDWLNLVFKWILFGQRPYWWVLDTDYYSNTSVPLIKQFPVTCETGPGSPSGHA

MGTAGVYYVMVTSTLSIFQGKIKPTYRFRCLNVILWLGFWAVQLNVCLSRIYLAAHFPHQ

VVAGVLSGIAVAETFSHIHSIYNASLKKYFLITFFLFSFAIGFYLLLKGLGVDLLWTLEK

AQRWCEQPEWVHIDTTPFASLLKNLGTLFGLGLALNSSMYRESCKGKLSKWLPFRLSSIV

ASLVLLHVFDSLKPPSQVELVFYVLSFCKSAVVPLASVSVIPYCLAQVLGQPHKKSL

6. Gene Sequence

>NC_000017.11:42900799-42914438 Homo sapiens chromosome 17, GRCh38.p14 Primary Assembly

AGCAGAGCAATCACCACCAAGCCTGGAATAACTGCAAGGGCTCTGCTGACATCTTCCTGAGGTGCCAAGG

AAATGAGGATGGAGGAAGGAATGAATGTTCTCCATGACTTTGGGATCCAGTCAACACATTACCTCCAGGT

GAATTACCAAGACTCCCAGGACTGGTTCATCTTGGTGTCCGTGATCGCAGACCTCAGGAATGCCTTCTAC

GTCCTCTTCCCCATCTGGTTCCATCTTCAGGAAGCTGTGGGCATTAAACTCCTTTGGGTAGCTGTGATTG

GAGACTGGCTCAACCTCGTCTTTAAGTGGTAAGAACCATATAGAGAGGAGATCAGCAAGAAAAGAGGCTG

GCATTCGCTCTCGCAATGTCTGTCCATCAGAAGTTGCTTTCCCCAGGCTATTCAGGAAGCCACGGGCTAC

TCATGCTTCCAACCCCTCTCTCTGACTTTGGATCATCTACATAAAGGGGGAAGACAGAAAAAATCCTACC

AGTGAGTTGAAAATACAGGAAAGCCTATTTCATATGGGTTAAAGGGTAGGACAGTTGAATTTCGTGAAAA

GTCTGAGTTATATAGGCTTTGAGCAAAGAGTTTTATTAGTATGAAGCAGAAGAGGTAACATAAAGAAAGA

TGTATGGGGCCAGGCATGGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCGAATCA

CTCCTGGGTGAACTCAGGAGTTCAAGACCAGCCTGGGCAACATGGCGAAACTCCATCTCTACAAAAACAT

TACGAAAATTAGCTGGGCGTGTTGGTGCTGTAGTCCCAGCTACTCAGGAGGCTGAGGTGAGAGGCGGAGG

AGGTTGCAGTGAGTCAAGATCATGCCACTGCACTCCAGCCTGGGCAACAGAGTAAGACCCTGTCTCAAAA

AAAAAAAAAAGATAGATGATGTATGCTGTATGAAAAAAGGAAACACACAGATGATTCAACAGCCTGTTTT

GTGGGGTAATGAAAAGTCACCCTGGGAACTGGGCTCCAGCCCTCGTTCTGCCACCCACCAACTACATGTC

CTTGGCAAGTCATATCAATTATCTGAGTTTCTGTTTTATAATCTACAAATAGGTTATCTCTGGCAGCTTA

ATAATAATCAGGGTTAACATTTATTAAACAGTGTGTGCCAGTCCATGTGCTATGTGCTTTTCTGTGAGGT

AGTTACTGCTATTTACAGAAACAGTAGATGCAGAGACCAAGGTGCTGAGTTAAATGATTAGGCCAACAAG

GTTAGTACATGCCGAGCCAGGATGGAAGCCCAGGTAGGCAGGCTGGCTTCCGCGGCAATGCTCTTATGAA

CTATGTTACGTCCAGTGCTGATAAACTGACTCTCTGGGGAGCAGGGGAAAGCCCTGAGTTTAGCATTTGC

CAATTTCTATCACGTAAACATTCCCATTCTGGCCACTTTCTTTCTTTCTTTCTTTTGTTTGTTTGTTTGA

GATGGAGTCTCGCACTGTTGCCTGGCTGGAGTGCAATGGTGCAATCTCAGCTCACTGCAACCTCTGCCTC

TCCGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGTGCCCGCCACCATGCCC

AGCTAATTTTTTTTGTATTTTTAGTAGAGACATGGTTTCACTATGTTGACTAGGCTGGTCTCGAACTCCT

GACCTCATGATCTGCCTGCCTTGGCCTCCCTAAGTGCTAGGATTACAGGCGTGAGCCACTACACCCAGCC

GCATGATTCTAAAAAATAAAAAGATGAAGTGTTATTCCAAACATCTGATCTCCATTGAAGAACCATGCAA

TCTCTCTGGGTTGATAGAGGCCAGAGTTAGTGGCTCTCCCTGATTTCGGTGAGAAATCACTATTCCACCA

TCACGGGATAAAAGGCATCCTGACTGGCGGTTGACACCTATTTCCACAGTGAAAGATATATCTAGTACTT

TTAAAGGGGAAGTGGTTTGTCTGAGATACTCTGTTTCAAAGTAGAGAGGATACAGAACAAGCATCTGAAG

CTATATACATCCTTACAGAGAGCAATTCTGATGGAAATGCAGGCCATGTTTCCCTGGGGGGGGCTCGTCC

TAGGGGCTGGAGTGCATTCTCTGATGTCAGAGGAAATGCAAGATTCCCTGAGGCCTGAGGGAACCCATGG

TATATGCAAGTCCAAGTTTCAAACTGTAGTTCCATATGCATTCTTCCAGGACAAATACTTCTTGAGGTTA

AAAAAAAAAAGTCACATAGCTGCCATTTTATGGATTTCAGGATTTTTTTTTTTTTTTTTTTGAGATGGAG

TCTTGCTCTGTCACCCAGCCTGTAGTGCAGTGGCATAATCTCGGCTCACGGCAACCTCCGCCTCCCAGGT

TCAAGCGATTCTCTTGCCTTAGCCTCCCGAGTAGCTGGGATTACAGTCACGCACCACCACATCTGGCTAA

TTCTTTATATTTTTTGGTAGAAACGGTGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCA

TGTGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGAGATTACAGGTGTGAGCCACCGCGCCTGCCTGGAGT

TCAGAATCTTGGGCTTCATTATTTGTGTTTAAATAGATCATACAGTCAGGCACGGTGGCTCATGCCTGTA

ATCCCAGCACTTTGGGAGGCTGAGGTGGGAGGATTGCCTGAGTTCAGGAGATGGAGACCAGCCTGGGCAA

CATGGTGAAACCCCGTCTCTACTAAAAATACAAAAACTAGCTGGATGTGGTGGCACACACCTGTAGTCCC

AGCTATTCAGGAGGCTGAGGTGGGAGGATCCCAGGAGGTAGAGGTCACAATGAGCCGAGATTGCGCCACT

GCACTCCAGGCTGGGTTACTGAGCCAGATCCTGTCTCAAAAAAAAAAAAGATAATACATTCAAACAGTTC

AAAATGCAAAAGTTACATACATAAGGAAGTGTCATGAAATATCTCCCTCTCACACTTCTCCCCAGCCACC

CAGTTCTCCCTTCTAGAGGCAACATGTGAAATCCTTCTCAGGCTACACTCTTCTTGAAGGTGTAGGCTTT

GGGCAAAAGCATTCATTCAGTAACCCCAGAAACTTGTTCTGTTTTTCCATAGGATTCTCTTTGGACAGCG

TCCATACTGGTGGGTTTTGGATACTGACTACTACAGCAACACTTCCGTGCCCCTGATAAAGCAGTTCCCT

GTAACCTGTGAGACTGGACCAGGTAAGCGTCCCAGCCCCTGCAGACAGAAGCTGAGTGGACCTCGTTTAC

CTGTTATGGATGAAACTGACCTTGAGGGGACATGAGGAGAGCCATTCCTTTGTACTTTTGTCATGCTCTT

CAATTGGCACAAATTAATTCACTTCTGCAATACTTTCCTGAATAGCACAGTAGTATTGGAAATCTGCCTA

TTACAGAACCTGGATGGAGTCCAGAGAGGCACGGGCATCCATGGGCAAAGGGCTCGTGAGAGTCACCGCC

CTGCAGCGCTGTGTCCTGAGAAAGGAGGGGGCAGAAGCCTGAGCTTCTGGGGGTCCTTCCCAATGGCCTG

GCCCACTGGATGTGCCCTCCTGAGCTGACCGTCCAATCCCTTGCCCTCTCTGTGCCTACGTTTTATTAGT

TACAGCCAGATGGTTACTGTCAAATCAAATGATAGATTTCATTTTCAGTATGTAATAGGAAGCCCCTCCC

TCACCCTAAAGTCTCAGCTGCCCTCTAAGACTAGTACTCTCTAAGGTACTAGTATCCCTTCCTCAGAGAC

CCTTTCCCTGACCCCAAAACTAGGGAAGGTCCCTTAGTTATTTGCTCTCACAGACCACGCATTTACCTCA

GAGCATATTCACTCATTCAGCTGTTACTTACCAAGCACCTACTGGGAGCTATACACTGTTCTATGTGCTA

GGGATACCTCTGTCAGTGAACAACACAGACACAAAGATCCCTGCCCTTGTGGAGCTGAAATCTGAATAGA

GGAGGTGAAATATACAAAAATTATAATAAATAAGTAAACTAGGCCAGTTGTGGTTGCTCATGCCTGTAAT

CCCAGCACTTTGGGAAGCCAAGGTAGGTAGATCACCTGAGGTCAGGAGTTCAAAACCAGCCTGGCCAACA

TTGCAAAATCCTGTCTTTACTAAAAATGGAAAAATTGGTCAGGCGTGATGGCACACGCCTGTAGTCTCAG

CTACCTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAGGCAGAGGTTGCAGTGAACCGAGATCGG

ACCACTGCACTCCAGCCTGAATGACAGAACGAGACTCTGTCTCAAAAAAAAAGTAAACTATTAATATGTA

GGATAGGCCAGGCACGGTGGCTCACCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGTGGATCACCT

GAGGTGAGGAGTTCAAGACCAGCCTGGCCAACATGGCAAAACCCTGTCTCTACTAAAAATACAAAAATTA

GCTGGGTGTCCTGGTGCATGCCTGTAATCTGAGCTACTCAGGAGGCTAAGGCAGGAGAATCGCTTGAACC

TGGGAGGTGGTGAGCCAAGATTGCGCCATTGCACTCCAGCCTGGGCGACAAAATGAGACACCATCTGAAA

AAAAAAAAAAAATATATATATATATACACACACACACACACACACACACACACACACATATAATACTAGA

AAATGATTGTTTATAGGCAAAAAAAAAAAAAAAGAAGAAGAAGAAGAAAAGGAAAGGAGAAGGAAAGAAG

GACCAAACATCTTTTGTAGAAATATGTTTGCTTTCATCATAACAGCTTGTTATCAAGGATGAATTTCTCC

CTGAAATTAATGGAGGCACAGACTGGAAAGTTTAAAGTGGCTTTAAGAGGTTATTTTATTTAGTCCTCTG

TCTTAATAGAAGCAAATTATTATCTCTGCTCCTTAGGTAGAGTAGCTAAGGCTCAGAAAGTAGGCCGGGC

GCGGTGGCTCACGCCTGTAATCCTAGCACTTTGGGAGGCCAACGCAGGTGGATCACCTGAGGTCAGGAGT

TTGAGACCAGCCTGGCCAACATGGTGAAACCTCGTCACTAATAAAAAAATACAAAAACTTAGCCAGGCAT

GGTGGCGGGCGCCTGTAATCCCAGCTACCCAGGAGGCTGCGGCAGGAGAATCACTTCAACCCGGGAGGCA

GAGGTTGCAGTGAGCTGAAATCACACCACTGCACTCCAGCCTTGGTGACAGAGAAAGATTCTGTCAGGAA

AAAAAAAAAAAAGTTTAAATGAATTACCCAAGGTATATAATTGTTAGTGTTAGAAGGAAGAAGAAGGGAG

GGAGGAAGGAAGGGAGAAAGAAAGGGAAGGAGGAAGGGAGGGAGGGAAGAAAGCCTTTATTTATCTATGG

GGTTCCCTGGAAAGCAGGCTGAAATGGAGATTCACGTGCAGGAGTTTAGATACTCTGGGGAACTATACTT

GTAGAAGGGAAGGAACAGGAACAGGGCAGAAGGAGAGGTCCGGTTGTGATTCTGCCTCATCCAACCCCAC

AGCGAGCTCTGAAGCTGGGGATGGCTCCTCAGAGTTGGTCCAAGTTGGGACAAGGGAATCAGACCCTGGG

GAGAGCGTAACCTTGATCAAGGCGACTCTCTTTAGCCCAGGGCAATGCCAGGAGAAGGCTGAGAGCAGAA

AGCCATCTACCATCACACTCTCAACAGCTACGAAATAAGTCCTGCAGTTCAGGAGGGAGGTCTGGGCGGC

ACATCTCAGGACCCTCTATCTCTCAGGGTAGAGGAATTAAGAATGGGATGGGAACCAGACGGGCCATGGT

GGCTCACACCTATAATCCCAACACTTTGGGAGGCCAAGGGTAGGAGGATTGCTTGAGCCCAAGAGTTCAA

AACCAGCCTGGGCAAAAACAATCAAACAAACAAACAAAACAC

7. Motif

Pfam (3 motifs)

Pfam	Position(Independent E-value)	Description
PAP2	59..198(2.1e-17)	PF01569, PAP2 superfamily
ATPase_gene1	207..228(0.099	PF09527, Putative F0F1-ATPase subunit Ca2+/Mg2+ transporter
DUF373	207..271(0.25)	PF04123, Domain of unknown function (DUF373)

8. Evolutionary Relationship

Evolutionary analysis by Maximum Likelihood method

The evolutionary history was inferred by using the Maximum Likelihood method and JTT matrix-based model [1]. The tree with the highest log likelihood (-5215.52) is shown. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using a JTT model, and then selecting the topology with superior log likelihood value. The proportion of sites where at least 1 unambiguous base is present in at least 1 sequence for each descendent clade is shown next to each internal node in the tree. This analysis involved 100 amino acid sequences. There were a total of 374 positions in the final dataset.

9. SNP

SNP	COORDINATE	AMINO ACID CHANGE	GENE ID	TRANSCRIPT ID	PROTEIN ID	SIFT SCORE	SIFT MEDIAN	SIFT PREDICTION
rs1801175	41055964	R83C	ENSG00000131482	ENST00000253801	ENSP00000253801	0	2.48	DELETERIOUS
rs1801175	41055964	R83C	ENSG00000131482	ENST00000585489	ENSP00000466202	0	2.64	DELETERIOUS
rs1801175	41055964	R83C	ENSG00000131482	ENST00000592383	ENSP00000465958	0	3.07	DELETERIOUS
rs1801176	41055965	R83H	ENSG00000131482	ENST00000253801	ENSP00000253801	0	2.48	DELETERIOUS
rs1801176	41055965	R83H	ENSG00000131482	ENST00000585489	ENSP00000466202	0	2.64	DELETERIOUS
rs1801176	41055965	R83H	ENSG00000131482	ENST00000592383	ENSP00000465958	0	3.07	DELETERIOUS
rs80356482	41061435	G188R	ENSG00000131482	ENST00000253801	ENSP00000253801	0	2.48	DELETERIOUS
rs80356482	41061435	R162T	ENSG00000131482	ENST00000592383	ENSP00000465958	0	4.32	DELETERIOUS (WARNING LOW CONFIDENCE)
rs80356484	41063017	L216L	ENSG00000131482	ENST00000253801	ENSP00000253801	1	2.48	TOLERATED
rs80356484	41063017		ENSG00000131482	ENST00000585489	ENSP00000466202
rs80356484	41063017		ENSG00000131482	ENST00000592383	ENSP00000465958
rs80356487	41063408	Q347*	ENSG00000131482	ENST00000253801	ENSP00000253801
rs80356487	41063408		ENSG00000131482	ENST00000585489	ENSP00000466202
rs104894563	41063252	R295C	ENSG00000131482	ENST00000253801	ENSP00000253801	0.002	2.47	DELETERIOUS
rs104894563	41063252		ENSG00000131482	ENST00000585489	ENSP00000466202
rs104894563	41063252		ENSG00000131482	ENST00000592383	ENSP00000465958
rs104894565	41053006	D38V	ENSG00000131482	ENST00000253801	ENSP00000253801	0	2.47	DELETERIOUS
rs104894565	41053006	D38V	ENSG00000131482	ENST00000592383	ENSP00000465958	0	3.06	DELETERIOUS
rs104894565	41053006	D38V	ENSG00000131482	ENST00000585489	ENSP00000466202	0.001	2.69	DELETERIOUS
rs104894569	41061424	W158*	ENSG00000131482	ENST00000592383	ENSP00000465958
rs104894569	41061424	G184E	ENSG00000131482	ENST00000253801	ENSP00000253801	0.003	2.48	DELETERIOUS
rs104894571	41061370	V166G	ENSG00000131482	ENST00000253801	ENSP00000253801	0	2.49	DELETERIOUS
rs104894571	41061370	C140W	ENSG00000131482	ENST00000592383	ENSP00000465958	0.029	3.43	DELETERIOUS
rs142917638	41055975	W86*	ENSG00000131482	ENST00000253801	ENSP00000253801
rs142917638	41055975	W86*	ENSG00000131482	ENST00000585489	ENSP00000466202
rs142917638	41055975	W86*	ENSG00000131482	ENST00000592383	ENSP00000465958
rs373345919	41061381	R170*	ENSG00000131482	ENST00000253801	ENSP00000253801
rs373345919	41061381	T144M	ENSG00000131482	ENST00000592383	ENSP00000465958	0.003	3.43	DELETERIOUS
rs387906505	41063391	I341N	ENSG00000131482	ENST00000253801	ENSP00000253801	0.002	2.49	DELETERIOUS
rs387906505	41063391		ENSG00000131482	ENST00000585489	ENSP00000466202

Evolutionary analysis of coding SNPs

SNP	Substitution	Probability of deleterious effect	Prediction Score
rs1801175	R83C	probably damaging	0.89
rs1801175	R83C	probably damaging	0.89
rs1801175	R83C	probably damaging	0.89
rs1801176	R83H	probably damaging	0.89
rs1801176	R83H	probably damaging	0.89
rs1801176	R83H	probably damaging	0.89
rs80356482	G188R	probably damaging	0.89
rs80356484	L216L	probably damaging	0.78
rs80356484	R295C	probably benign	0.13
rs80356484	D38V	probably damaging	0.89
rs80356487	D38V	probably damaging	0.89
rs80356487	D38V	probably damaging	0.89
rs104894563	G184E	probably damaging	0.89
rs104894563	V166G	possibly damaging	0.5
rs104894565	I341N	probably benign	0.13

Provean

SNP Id’s	Amino Acid Change		probability	Prediction	Score
rs1801175	R83C	Deleterious		Damaging	-6.09
rs1801176	R83H	Deleterious		Damaging	-4.4
rs80356482	G188R	Deleterious		Damaging	-6.92
rs80356484	L216L	Neutral		Tolerated	0
rs104894563	R295C	Deleterious		Damaging	-3.43
rs104894565	D38V	Deleterious		Damaging	-8.11
rs104894569	G184E	Deleterious		Damaging	-6.55
rs104894571	V166G	Deleterious		Damaging	-4.58
rs387906505	I341N	Deleterious		Damaging	-4.51

SNAP2

Wildetype Amino Acid	Position	Variant Amino Acid	Predicted Effect	Score	Expected Accurancy
R	83	C	Effect	96	98%
R	83	H	Neutral	46	78%
G	188	R	Effect	56	98%
L	216	L	Neutral	85	76%
R	295	C	Effect	96	98%
D	38	V	Neutral	86	78%
G	184	E	Effect	56	98%
V	166	G	Effect	24	76%
I	341	N	Effect	98	98%

Polyphene 2

Rs_Id	Mutation Probability	Score
rs1801175	Probably damaging	0.999
rs1801176	Possibly damaging	1.00
rs80356482	Probably damaging	0.98
rs80356484	Probably damaging	0.98
rs104894563	Possibly damaging	1.00
rs104894565	Probably damaging	0.999
rs104894569	Possibly damaging	1.00
rs104894571	Probably damaging	0.98
rs387906505	Probably damaging	0.98

Hope Prediction

Rs_ids	Mutation	Mapping issue	AA variant	Function. Impact	Score
rs1801175	Glucose-6-phosphatase_R83C		R83C	High	5.16
rs1801176	Glucose-6-phosphatase_R83H	Uniprot residue: R	R83H
rs80356482	Glucose-6-phosphatase_G188R	Uniprot residue: I	G188R
rs80356484	Glucose-6-phosphatase_L216L		L216L	High	4.653
rs104894563	Glucose-6-phosphatase_R295C		R295C	Medium	2.342
rs104894565	Glucose-6-phosphatase_D38V		D38V	Medium	3.421
rs104894569	Glucose-6-phosphatase_G184E	Uniprot residue: C	G184E
rs104894571	Glucose-6-phosphatase_V166G		V166G	Low	1.062
rs387906505	Glucose-6-phosphatase_I341N	Uniprot residue: G	I341N

10. Active Site

Nil

11. Primary Sequence Analysis

Number of amino acids

357

Molecular weight

40483.55

Theoretical pI

8.72

Amino acid composition

Ala (A) 21 5.9%

Arg (R) 8 2.2%

Asn (N) 10 2.8%

Asp (D) 10 2.8%

Cys (C) 7 2.0%

Gln (Q) 15 4.2%

Glu (E) 10 2.8%

Gly (G) 22 6.2%

His (H) 11 3.1%

Ile (I) 20 5.6%

Leu (L) 51 14.3%

Lys (K) 17 4.8%

Met (M) 5 1.4%

Phe (F) 25 7.0%

Pro (P) 16 4.5%

Ser (S) 30 8.4%

Thr (T) 15 4.2%

Trp (W) 13 3.6%

Tyr (Y) 16 4.5%

Val (V) 35 9.8%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

Total number of negatively charged residues (Asp + Glu)

Total number of positively charged residues (Arg + Lys)

Formula

C₁₉₁₇H₂₈₉₂N₄₅₈O₄₈₄S₁₂

Total number of atoms

5763

Instability index(II)

36.80

Stability

stable

Aliphatic index(AI)

111.88

GRAVY

0.474

12. Secondary structure analysis

Alpha helix (Hh) : 221 is 61.90%

310 helix (Gg) : 0 is 0.00%

Pi helix (Ii) : 0 is 0.00%

Beta bridge (Bb) : 0 is 0.00%

Extended strand (Ee) : 22 is 6.16%

Beta turn (Tt) : 0 is 0.00%

Bend region (Ss) : 0 is 0.00%

Random coil (Cc) : 114 is 31.93%

Ambiguous states (?) : 0 is 0.00%

Other states : 0 is 0.00%

Secondary Structure Prediction

Amino Acid Type

13. Enzymes

Name of enzyme	No. of cleavages
Arg-C proteinase	8
Asp-N endopeptidase	10
Asp-N endopeptidase + N-terminal Glu	20
BNPS-Skatole	13
CNBr	5
Caspase1	1
Chymotrypsin-high specificity (C-term to [FYW], not before P)	51
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	115
Clostripain	8
Formic acid	10
Glutamyl endopeptidase	10
Iodosobenzoic acid	13
LysC	17
LysN	17
NTCB (2-nitro-5-thiocyanobenzoic acid)	7
Pepsin (pH1.3)	111
Pepsin (pH>2)	142
Proline-endopeptidase	2
Proteinase K	206
Staphylococcal peptidase I	9
Thermolysin	144
Trypsin	22

14. Epitope

Rank	Start position	Sequence	Score	Prediction
1	241	AQRWCEQPEWVHIDTT	0.94	Epitope
2	109	CETGPGSPSGHAMGTA	0.93	Epitope
3	139	QGKIKPTYRFRCLNVI	0.90	Epitope
4	118	GHAMGTAGVYYVMVTS	0.89	Epitope
4	11	FGIQSTHYLQVNYQDS	0.89	Epitope
5	85	YWWVLDTDYYSNTSVP	0.83	Epitope
5	248	PEWVHIDTTPFASLLK	0.83	Epitope
6	91	TDYYSNTSVPLIKQFP	0.81	Epitope
7	337	SVSVIPYCLAQVLGQP	0.77	Epitope
8	186	LSGIAVAETFSHIHSI	0.76	Epitope
9	76	KWILFGQRPYWWVLDT	0.75	Epitope
9	168	LSRIYLAAHFPHQVVA	0.75	Epitope
10	155	LWLGFWAVQLNVCLSR	0.74	Epitope
11	318	VELVFYVLSFCKSAVV	0.73	Epitope

15. Protein-Protein Interaction

No of Nodes	11
No of Edges	46
Avg node degree	8.36
avg. local clustering coefficient	0.894
expected number of e, dges	13
p-value	8.45e-13
Protein – Protein Interaction network

16. MRNA

RNA Base Pairing Probability Plot