Disease Name: H syndrome
>sp|Q9BZD2|S29A3_HUMAN Equilibrative nucleoside transporter 3 OS=Homo sapiens OX=9606 GN=SLC29A3 PE=1 SV=3
MAVVSEDDFQHSSNSTYRTTSSSLRADQEALLEKLLDRPPPGLQRPEDRFCGTYIIFFSL
GIGSLLPWNFFITAKEYWMFKLRNSSSPATGEDPEGSDILNYFESYLAVASTVPSMLCLV
ANFLLVNRVAVHIRVLASLTVILAIFMVITALVKVDTSSWTRGFFAVTIVCMVILSGAST
VFSSSIYGMTGSFPMRNSQALISGGAMGGTVSAVASLVDLAASSDVRNSALAFFLTATVF
LVLCMGLYLLLSRLEYARYYMRPVLAAHVFSGEEELPQDSLSAPSVASRFIDSHTPPLRP
ILKKTASLGFCVTYVFFITSLIYPAICTNIESLNKGSGSLWTTKFFIPLTTFLLYNFADL
CGRQLTAWIQVPGPNSKALPGFVLLRTCLIPLFVLCNYQPRVHLKTVVFQSDVYPALLSS
LLGLSNGYLSTLALLYGPKIVPRELAEATGVVMSFYVCLGLTLGSACSTLLVHLI
>NM_001174098.1 Homo sapiens solute carrier family 29 member 3 (SLC29A3), transcript variant 2, mRNA
AAGCCCAGTGGTCCTGGCCGTGCGCCGGAGGCAGCGGCGGCGTGGCGCAGCGGCGACATGGCCGTTGTCT
CAGAGGACGACTTTCAGCACAGTTCAAACTCCACCTACAGAACCACAAGCAGCAGTCTCCGAGCTGACCA
GGAGGCACTGCTTGAGAAGCTGCTGGACCGCCCGCCCCCTGGCCTGCAGAGGCCCGAGGACCGCTTCTGT
GGCACATACATCATCTTCTTCAGCCTGGGCATTGGCAGTCTACTGCCATGGAACTTCTTTATCACTGCCA
AGGAGTACTGGATGTTCAAACTCCGCAACTCCTCCAGCCCAGCCACCGGGGAGGACCCTGAGGGCTCAGA
CATCCTGAACTACTTTGAGAGCTACCTTGCCGTTGCCTCCACCGTGCCCTCCATGCTGTGCCTGGTGGCC
AACTTCCTGCTTGTCAACAGGGTTGCAGTCCACATCCGTGTCCTGGCCTCACTGACGGTCATCCTGGCCA
TCTTCATGGTGATAACTGCACTGGTGAAGGTGGACACTTCCTCCTGGACCCGTGGCTTTTTTGCGGTCAC
CATTGTCTGCATGGTGATCCTCAGCGGTGCCTCCACTGTCTTCAGCAGCAGCATCTACGGCATGACCGGC
TCCTTTCCTATGAGGAACTCCCAGGCACTGATATCAGGAGGAGCCATGGGCGGGACGGTCAGCGCCGTGG
CCTCATTGGTGGACTTGGCTGCATCCAGTGATGTGAGGAACAGCGCCCTGGCCTTCTTCCTGACGGCCAC
TGTCTTCCTCGTGCTCTGCATGGGACTCTACCTGCTGCTGTCCAGGCTGGAGTATGCCAGGTGAAGGTAC
TACATGAGGCCTGTTCTTGCGGCCCATGTGTTTTCTGGTGAAGAGGAGCTTCCCCAGGACTCCCTCAGTG
CCCCTTCGGTGGCCTCCAGATTCATTGATTCCCACACACCCCCTCTCCGCCCCATCCTGAAGAAGACGGC
CAGCCTGGGCTTCTGTGTCACCTACGTCTTCTTCATCACCAGCCTCATCTACCCCGCCATCTGCACCAAC
ATCGAGTCCCTCAACAAGGGTTCGGGCTCACTGTGGACCACCAAGTTTTTCATCCCCCTCACTACCTTCC
TCCTGTACAACTTTGCTGACCTATGTGGCCGGCAGCTCACCGCCTGGATCCAGGTGCCAGGGCCCAATAG
CAAGGCGCTCCCAGGGTTCGTGCTCCTCCGGACCTGCCTCATCCCCCTCTTCGTGCTCTGTAACTACCAG
CCCCGCGTCCACCTGAAGACTGTGGTCTTCCAGTCCGATGTGTACCCCGCACTCCTCAGCTCCCTGCTGG
GGCTCAGCAACGGCTACCTCAGCACCCTGGCCCTCCTCTACGGGCCTAAGATTGTGCCCAGGGAGCTGGC
TGAGGCCACGGGAGTGGTGATGTCCTTTTATGTGTGCTTGGGCTTAACACTGGGCTCAGCCTGCTCTACC
CTCCTGGTGCACCTCATCTAGAAGGGAGGACACAAGGACATTGGTGCTTCAGAGCCTTTGAAGATGAGAA
GAGAGTGCAGGAGGGCTGGGGGCCATGGAGGAAAGGCCTAAAGTTTCACTTGGGGACAGAGAGCAGAGCA
CACTCGGGCCTCATCCCTCCCAAGATGCCAGTGAGCCACGTCCATGCCCATTCCGTGCAAGGCAGATATT
CCAGTCATATTAACAGAACACTCCTGAGACAGTTGAAGAAGAAATAGCACAAATCAGGGGTACTCCCTTC
ACAGCTGATGGTTAACATTCCACCTTCTTTCTAGCCCTTCAAAGATGCTGCCAGTGTTCGCCCTAGAGTT
ATTACAAAGCCAGTGCCAAAACCCAGCCATGGGCTCTTTGCAACCTCCCAGCTGCGCTCATTCCAGCTGA
CAGCGAGATGCAAGCAAATGCTCAGCTCTCCTTACCCTGAAGGGGTCTCCCTGGAATGGAAGTCCCCTGG
CATGGTCAGTCCTCAGGCCCAAGACTCAAGTGTGCACAGACCCCTGTGTTCTGTGGGTGAACAACTGCCC
ACTAACCAGACTGGAAAACCCAGAAAGATGGGCCTTCCATGAATGCTTCATTCCAGAGGGACCAGAGGGC
CTCCCTGTGCAAGGGATCAAGCATGTCTGGCCTGGGTTTTCAAAAAAAGAGGGATCCTCATGACCTGGTG
GTCTATGGCCTGGGTCAAGATGAGGGTCTTTCAGTGTTCCTGTTTACAACATGTCAAAGCCATTGGTTCA
AGGGCGTAATAAATACTTGCGTATTCAATGTAAAAAAAAAAAA
Pfam (1 motif)
|
Pfam |
Position(Independent E-value) |
Description |
|
Nucleoside_tran |
172..472(7.3e-63) |
PF01733, Nucleoside transporter |
Evolutionary analysis by Maximum Likelihood method
The evolutionary history was inferred by using the Maximum Likelihood method and JTT matrix-based model [1]. The tree with the highest log likelihood (-12248.05) is shown. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using a JTT model, and then selecting the topology with superior log likelihood value. The proportion of sites where at least 1 unambiguous base is present in at least 1 sequence for each descendent clade is shown next to each internal node in the tree. This analysis involved 100 amino acid sequences. There were a total of 641 positions in the final dataset.
Nil
Nil
|
|
|
|
Number of amino acids
|
475 |
|
Molecular weight
|
51814.57
|
|
Theoretical pI
|
7.95
|
|
Amino acid composition |
Ala (A) 38 8.0% Arg (R) 20 4.2% Asn (N) 14 2.9% Asp (D) 14 2.9% Cys (C) 11 2.3% Gln (Q) 9 1.9% Glu (E) 15 3.2% Gly (G) 28 5.9% His (H) 6 1.3% Ile (I) 24 5.1% Leu (L) 70 14.7% Lys (K) 11 2.3% Met (M) 11 2.3% Phe (F) 30 6.3% Pro (P) 25 5.3% Ser (S) 53 11.2% Thr (T) 33 6.9% Trp (W) 5 1.1% Tyr (Y) 18 3.8% Val (V) 40 8.4% Pyl (O) 0 0.0% Sec (U) 0 0.0%
(B) 0 0.0% (Z) 0 0.0% (X) 0 0.0% |
|
Total number of negatively charged residues (Asp + Glu)
|
29
|
|
Total number of positively charged residues (Arg + Lys)
|
31
|
|
Formula
|
C2379H3722N586O661S22 |
|
Total number of atoms()
|
7370
|
|
Instability index(II)
|
39.90 |
|
Stability |
stable
|
|
Aliphatic index(AI)
|
109.60 |
|
GRAVY
|
0.556 |
|
Alpha helix (Hh) : 198 is 41.68% 310 helix (Gg) : 0 is 0.00% Pi helix (Ii) : 0 is 0.00% Beta bridge (Bb) : 0 is 0.00% Extended strand (Ee) : 73 is 15.37% Beta turn (Tt) : 0 is 0.00% Bend region (Ss) : 0 is 0.00% Random coil (Cc) : 204 is 42.95% Ambiguous states (?) : 0 is 0.00% Other states : 0 is 0.00% |
Secondary Structure Prediction
Amino Acid Type
|
Name of enzyme |
No. of cleavages |
|
Arg-C proteinase |
20 |
|
Asp-N endopeptidase |
14 |
|
Asp-N endopeptidase + N-terminal Glu |
29 |
|
BNPS-Skatole |
5 |
|
CNBr |
11 |
|
Chymotrypsin-high specificity (C-term to [FYW], not before P) |
49 |
|
Chymotrypsin-low specificity (C-term to [FYWML], not before P) |
133 |
|
Clostripain |
20 |
|
Formic acid |
14 |
|
Glutamyl endopeptidase |
15 |
|
Iodosobenzoic acid |
5 |
|
Hydroxylamine |
14 |
|
LysC |
11 |
|
LysN |
11 |
|
NTCB (2-nitro-5-thiocyanobenzoic acid) |
11 |
|
Pepsin (pH1.3) |
150 |
|
Pepsin (pH>2) |
176 |
|
Proline-endopeptidase |
3 |
|
Proteinase K |
273 |
|
Staphylococcal peptidase I |
13 |
|
Thermolysin |
193 |
|
Trypsin |
28 |
|
Rank |
Start position |
Sequence |
Score |
Prediction |
|
1 |
88 |
PATGEDPEGSDILNYF |
0.93 |
Epitope |
|
2 |
252 |
SRLEYARYYMRPVLAA |
0.89 |
Epitope |
|
3 |
183 |
SSSIYGMTGSFPMRNS |
0.88 |
Epitope |
|
4 |
427 |
GYLSTLALLYGPKIVP |
0.86 |
Epitope |
|
5 |
337 |
SGSLWTTKFFIPLTTF |
0.85 |
Epitope |
|
6 |
365 |
LTAWIQVPGPNSKALP |
0.84 |
Epitope |
|
6 |
291 |
IDSHTPPLRPILKKTA |
0.84 |
Epitope |
|
7 |
79 |
MFKLRNSSSPATGEDP |
0.83 |
Epitope |
|
8 |
41 |
PGLQRPEDRFCGTYII |
0.82 |
Epitope |
|
8 |
17 |
YRTTSSSLRADQEALL |
0.82 |
Epitope |
|
9 |
327 |
CTNIESLNKGSGSLWT |
0.80 |
Epitope |
|
9 |
157 |
TSSWTRGFFAVTIVCM |
0.80 |
Epitope |
|
10 |
269 |
VFSGEEELPQDSLSAP |
0.79 |
Epitope |
|
10 |
205 |
GAMGGTVSAVASLVDL |
0.79 |
Epitope |
|
10 |
112 |
TVPSMLCLVANFLLVN |
0.79 |
Epitope |
|
11 |
94 |
PEGSDILNYFESYLAV |
0.78 |
Epitope |
|
11 |
400 |
PRVHLKTVVFQSDVYP |
0.78 |
Epitope |
|
No of Nodes |
11 |
|
No of Edges |
14 |
|
Avg node degree |
2.55 |
|
avg. local clustering coefficient |
0.887 |
|
expected number of e, dges |
10 |
|
p-value |
0.142
|
|
Protein – Protein Interaction network |
|
RNA Base Pairing Probability Plot
