IGDRPT (Indian Genetic Disease Risk Prediction Tool)

Disease Name: Aniridia

1 .Inheritance

Autosomal Dominant

2. Gene Name

PAX6

3. Protein Name

Paired box protein Pax-6

4. Chromosomal Location

11p13

5. Protein Sequence

>sp|P26367|PAX6_HUMAN Paired box protein Pax-6 OS=Homo sapiens OX=9606 GN=PAX6 PE=1 SV=2

MQNSHSGVNQLGGVFVNGRPLPDSTRQKIVELAHSGARPCDISRILQVSNGCVSKILGRY

YETGSIRPRAIGGSKPRVATPEVVSKIAQYKRECPSIFAWEIRDRLLSEGVCTNDNIPSV

SSINRVLRNLASEKQQMGADGMYDKLRMLNGQTGSWGTRPGWYPGTSVPGQPTQDGCQQQ

EGGGENTNSISSNGEDSDEAQMRLQLKRKLQRNRTSFTQEQIEALEKEFERTHYPDVFAR

ERLAAKIDLPEARIQVWFSNRRAKWRREEKLRNQRRQASNTPSHIPISSSFSTSVYQPIP

QPTTPVSSFTSGSMLGRTDTALTNTYSALPPMPSFTMANNLPMQPPVPSQTSSYSCMLPT

SPSVNGRSYDTYTPPHMQTHMNSQPMGTSGTTSTGLISPGVSVPVQVPGSEPDMSQYWPR

6. Gene Sequence

>NM_000280.3 Homo sapiens paired box 6 (PAX6), transcript variant 1, mRNA

GGTGCATTTGCATGTTGCGGAGTGATTAGTGGGTTTGAAAAGGGAACCGTGGCTCGGCCTCATTTCCCGC

TCTGGTTCAGGCGCAGGAGGAAGTGTTTTGCTGGAGGATGATGACAGAGGTCAGGCTTCGCTAATGGGCC

AGTGAGGAGCGGTGGAGGCGAGGCCGGGCGCCGGCACACACACATTAACACACTTGAGCCATCACCAATC

AGCATAGGAATCTGAGAATTGCTCTCACACACCAACCCAGCAACATCCGTGGAGAAAACTCTCACCAGCA

ACTCCTTTAAAACACCGTCATTTCAAACCATTGTGGTCTTCAAGCAACAACAGCAGCACAAAAAACCCCA

ACCAAACAAAACTCTTGACAGAAGCTGTGACAACCAGAAAGGATGCCTCATAAAGGGGGAAGACTTTAAC

TAGGGGCGCGCAGATGTGTGAGGCCTTTTATTGTGAGAGTGGACAGACATCCGAGATTTCAGAGCCCCAT

ATTCGAGCCCCGTGGAATCCCGCGGCCCCCAGCCAGAGCCAGCATGCAGAACAGTCACAGCGGAGTGAAT

CAGCTCGGTGGTGTCTTTGTCAACGGGCGGCCACTGCCGGACTCCACCCGGCAGAAGATTGTAGAGCTAG

CTCACAGCGGGGCCCGGCCGTGCGACATTTCCCGAATTCTGCAGGTGTCCAACGGATGTGTGAGTAAAAT

TCTGGGCAGGTATTACGAGACTGGCTCCATCAGACCCAGGGCAATCGGTGGTAGTAAACCGAGAGTAGCG

ACTCCAGAAGTTGTAAGCAAAATAGCCCAGTATAAGCGGGAGTGCCCGTCCATCTTTGCTTGGGAAATCC

GAGACAGATTACTGTCCGAGGGGGTCTGTACCAACGATAACATACCAAGCGTGTCATCAATAAACAGAGT

TCTTCGCAACCTGGCTAGCGAAAAGCAACAGATGGGCGCAGACGGCATGTATGATAAACTAAGGATGTTG

AACGGGCAGACCGGAAGCTGGGGCACCCGCCCTGGTTGGTATCCGGGGACTTCGGTGCCAGGGCAACCTA

CGCAAGATGGCTGCCAGCAACAGGAAGGAGGGGGAGAGAATACCAACTCCATCAGTTCCAACGGAGAAGA

TTCAGATGAGGCTCAAATGCGACTTCAGCTGAAGCGGAAGCTGCAAAGAAATAGAACATCCTTTACCCAA

GAGCAAATTGAGGCCCTGGAGAAAGAGTTTGAGAGAACCCATTATCCAGATGTGTTTGCCCGAGAAAGAC

TAGCAGCCAAAATAGATCTACCTGAAGCAAGAATACAGGTATGGTTTTCTAATCGAAGGGCCAAATGGAG

AAGAGAAGAAAAACTGAGGAATCAGAGAAGACAGGCCAGCAACACACCTAGTCATATTCCTATCAGCAGT

AGTTTCAGCACCAGTGTCTACCAACCAATTCCACAACCCACCACACCGGTTTCCTCCTTCACATCTGGCT

CCATGTTGGGCCGAACAGACACAGCCCTCACAAACACCTACAGCGCTCTGCCGCCTATGCCCAGCTTCAC

CATGGCAAATAACCTGCCTATGCAACCCCCAGTCCCCAGCCAGACCTCCTCATACTCCTGCATGCTGCCC

ACCAGCCCTTCGGTGAATGGGCGGAGTTATGATACCTACACCCCCCCACATATGCAGACACACATGAACA

GTCAGCCAATGGGCACCTCGGGCACCACTTCAACAGGACTCATTTCCCCTGGTGTGTCAGTTCCAGTTCA

AGTTCCCGGAAGTGAACCTGATATGTCTCAATACTGGCCAAGATTACAGTAAAAAAAAAAAAAAAAAAAA

AAAGGAAAGGAAATATTGTGTTAATTCAGTCAGTGACTATGGGGACACAACAGTTGAGCTTTCAGGAAAG

AAAGAAAAATGGCTGTTAGAGCCGCTTCAGTTCTACAATTGTGTCCTGTATTGTACCACTGGGGAAGGAA

TGGACTTGAAACAAGGACCTTTGTATACAGAAGGCACGATATCAGTTGGAACAAATCTTCATTTTGGTAT

CCAAACTTTTATTCATTTTGGTGTATTATTTGTAAATGGGCATTTGTATGTTATAATGAAAAAAAGAACA

ATGTAGACTGGATGGATGTTTGATCTGTGTTGGTCATGAAGTTGTTTTTTTTTTTTTTAAAAAGAAAACC

ATGATCAACAAGCTTTGCCACGAATTTAAGAGTTTTATCAAGATATATCGAATACTTCTACCCATCTGTT

CATAGTTTATGGACTGATGTTCCAAGTTTGTATCATTCCTTTGCATATAATTAAACCTGGAACAACATGC

ACTAGATTTATGTCAGAAATATCTGTTGGTTTTCCAAAGGTTGTTAACAGATGAAGTTTATGTGCAAAAA

AGGGTAAGATATAAATTCAAGGAAGAAAAAAAGTTGATAGCTAAAAGGTAGAGTGTGTCTTCGATATAAT

CCAATTTGTTTTATGTCAAAATGTAAGTATTTGTCTTCCCTAGAAATCCTCAGAATGATTTCTATAATAA

AGTTAATTTCATTTATATTTGACAAGAATATAGATGTTTTATACACATTTTCATGCAATCATACGTTTCT

TTTTTGGCCAGCAAAAGTTAATTGTTCTTAGATATAGTTGTATTACTGTTCACGGTCCAATCATTTTGTG

CATCTAGAGTTCATTCCTAATCAATTAAAAGTGCTTGCAAGAGTTTTAAACTTAAGTGTTTTGAAGTTGT

TCACAACTACATATCAAAATTAACCATTGTTGATTGTAAAAAACCATGCCAAAGCCTTTGTATTTCCTTT

ATTATACAGTTTTCTTTTTAACCTTATAGTGTGGTGTTACAAATTTTATTTCCATGTTAGATCAACATTC

TAAACCAATGGTTACTTTCACACACACTCTGTTTTACATCCTGATGATCCTTAAAAAATAATCCTTATAG

ATACCATAAATCAAAAACGTGTTAGAAAAAAATTCCACTTACAGCAGGGTGTAGATCTGTGCCCATTTAT

ACCCACAACATATATACAAAATGGTAACATTTCCCAGTTAGCCATTTAATTCTAAAGCTCAAAGTCTAGA

AATAATTTAAAAATGCAACAAGCGATTAGCTAGGAATTGTTTTTTGAATTAGGACTGGCATTTTCAATCT

GGGCAGATTTCCATTGTCAGCCTATTTCAACAATGATTTCACTGAAGTATATTCAAAAGTAGATTTCTTA

AAGGAGACTTTCTGAAAGCTGTTGCCTTTTTCAAATAGGCCCTCTCCCTTTTCTGTCTCCCTCCCCTTTG

CACAAGAGGCATCATTTCCCATTGAACCACTACAGCTGTTCCCATTTGAATCTTGCTTTCTGTGCGGTTG

TGGATGGTTGGAGGGTGGAGGGGGGATGTTGCATGTCAAGGAATAATGAGCACAGACACATCAACAGACA

ACAACAAAGCAGACTGTGACTGGCCGGTGGGAATTAAAGGCCTTCAGTCATTGGCAGCTTAAGCCAAACA

TTCCCAAATCTATGAAGCAGGGCCCATTGTTGGTCAGTTGTTATTTGCAATGAAGCACAGTTCTGATCAT

GTTTAAAGTGGAGGCACGCAGGGCAGGAGTGCTTGAGCCCAAGCAAAGGATGGAAAAAAATAAGCCTTTG

TTGGGTAAAAAAGGACTGTCTGAGACTTTCATTTGTTCTGTGCAACATATAAGTCAATACAGATAAGTCT

TCCTCTGCAAACTTCACTAAAAAGCCTGGGGGTTCTGGCAGTCTAGATTAAAATGCTTGCACATGCAGAA

ACCTCTGGGGACAAAGACACACTTCCACTGAATTATACTCTGCTTTAAAAAAATCCCCAAAAGCAAATGA

TCAGAAATGTAGAAATTAATGGAAGGATTTAAACATGACCTTCTCGTTCAATATCTACTGTTTTTTAGTT

AAGGAATTACTTGTGAACAGATAATTGAGATTCATTGCTCCGGCATGAAATATACTAATAATTTTATTCC

ACCAGAGTTGCTGCACATTTGGAGACACCTTCCTAAGTTGCAGTTTTTGTATGTGTGCATGTAGTTTTGT

TCAGTGTCAGCCTGCACTGCACAGCAGCACATTTCTGCAGGGGAGTGAGCACACATACGCACTGTTGGTA

CAATTGCCGGTGCAGACATTTCTACCTCCTGACATTTTGCAGCCTACATTCCCTGAGGGCTGTGTGCTGA

GGGAACTGTCAGAGAAGGGCTATGTGGGAGTGCATGCCACAGCTGCTGGCTGGCTTACTTCTTCCTTCTC

GCTGGCTGTAATTTCCACCACGGTCAGGCAGCCAGTTCCGGCCCACGGTTCTGTTGTGTAGACAGCAGAG

ACTTTGGAGACCCGGATGTCGCACGCCAGGTGCAAGAGGTGGGAATGGGAGAAAAGGAGTGACGTGGGAG

CGGAGGGTCTGTATGTGTGCACTTGGGCACGTATATGTGTGCTCTGAAGGTCAGGATTGCCAGGGCAAAG

TAGCACAGTCTGGTATAGTCTGAAGAAGCGGCTGCTCAGCTGCAGAAGCCCTCTGGTCCGGCAGGATGGG

AACGGCTGCCTTGCCTTCTGCCCACACCCTAGGGACATGAGCTGTCCTTCCAAACAGAGCTCCAGGCACT

CTCTTGGGGACAGCATGGCAGGCTCTGTGTGGTAGCAGTGCCTGGGAGTTGGCCTTTTACTCATTGTTGA

AATAATTTTTGTTTATTATTTATTTAACGATACATATATTTATATATTTATCAATGGGGTATCTGCAGGG

ATGTTTTGACACCATCTTCCAGGATGGAGATTATTTGTGAAGACTTCAGTAGAATCCCAGGACTAAACGT

CTAAATTTTTTCTCCAAACTTGACTGACTTGGGAAAACCAGGTGAATAGAATAAGAGCTGAATGTTTTAA

GTAATAAACGTTCAAACTGCTCTAAGTAAAAAAATGCATTTTACTGCAATGAATTTCTAGAATATTTTTC

CCCCAAAGCTATGCCTCCTAACCCTTAAATGGTGAACAACTGGTTTCTTGCTACAGCTCACTGCCATTTC

TTCTTACTATCATCACTAGGTTTCCTAAGATTCACTCATACAGTATTATTTGAAGATTCAGCTTTGTTCT

GTGAATGTCATCTTAGGATTGTGTCTATATTCTTTTGCTTATTTCTTTTTACTCTGGGCCTCTCATACTA

GTAAGATTTTAAAAAGCCTTTTCTTCTCTGTATGTTTGGCTCACCAAGGCGAAATATATATTCTTCTCTT

TTTCATTTCTCAAGAATAAACCTCATCTGCTTTTTTGTTTTTCTGTGTTTTGGCTTGGTACTGAATGACT

CAACTGCTCGGTTTTAAAGTTCAAAGTGTAAGTACTTAGGGTTAGTACTGCTTATTTCAATAATGTTGAC

GGTGACTATCTTTGGAAAGCAGTAACATGCTGTCTTAGAAATGACATTAATAATGGGCTTAAACAAATGA

ATAGGGGGGTCCCCCCACTCTCCTTTTGTATGCCTATGTGTGTCTGATTTGTTAAAAGATGGACAGGGAA

TTGATTGCAGAGTGTCGCTTCCTTCTAAAGTAGTTTTATTTTGTCTACTGTTAGTATTTAAAGATCCTGG

AGGTGGACATAAGGAATAAATGGAAGAGAAAAGTAGATATTGTATGGTGGCTACTAAAAGGAAATTCAAA

AAGTCTTAGAACCCGAGCACCTGAGCAAACTGCAGTAGTCAAAATATTTATCTCATGTTAAAGAAAGGCA

AATCTAGTGTAAGAAATGAGTACCATATAGGGTTTTGAAGTTCATATACTAGAAACACTTAAAAGATATC

ATTTCAGATATTACGTTTGGCATTGTTCTTAAGTATTTATATCTTTGAGTCAAGCTGATAATTAAAAAAA

ATCTGTTAATGGAGTGTATATTTCATAATGTATCAAAATGGTGTCTATACCTAAGGTAGCATTATTGAAG

AGAGATATGTTTATGTAGTAAGTTATTAACATAATGAGTAACAAATAATGTTTCCAGAAGAAAGGAAAAC

ACATTTTCAGAGTGCGTTTTTATCAGAGGAAGACAAAAATACACACCCCTCTCCAGTAGCTTATTTTTAC

AAAGCCGGCCCAGTGAATTAGAAAAACAAAGCACTTGGATATGATTTTTGGAAAGCCCAGGTACACTTAT

TATTCAAAATGCACTTTTACTGAGTTTGAAAAGTTTCTTTTATATTTAAAATAAGGGTTCAAATATGCAT

ATTCAATTTTTATAGTAGTTATCTATTTGCAAAGCATATATTAACTAGTAATTGGCTGTTAATTTTATAG

ACATGGTAGCCAGGGAAGTATATCAATGACCTATTAAGTATTTTGACAAGCAATTTACATATCTGATGAC

CTCGTATCTCTTTTTCAGCAAGTCAAATGCTATGTAATTGTTCCATTGTGTGTTGTATAAAATGAATCAA

CACGGTAAGAAAAAGGTTAGAGTTATTAAAATAATAAACTGACTAAAATACTCATTTGAATTTATTCAGA

ATGTTCATAATGCTTTCAAAGGACATAGCAGAGCTTTTGTGGAGTATCCGCACAACATTATTTATTATCT

ATGGACTAAATCAATTTTTTGAAGTTGCTTTAAAATTTAAAAGCACCTTTGCTTAATATAAAGCCCTTTA

ATTTTAACTGACAGATCAATTCTGAAACTTTATTTTGAAAAGAAAATGGGGAAGAATCTGTGTCTTTAGA

ATTAAAAGAAATGAAAAAAATAAACCCGACATTCTAAAAAAATAGAATAAGAAACCTGATTTTTAGTACT

AATGAAATAGCGGGTGACAAAATAGTTGTCTTTTTGATTTTGATCACAAAAAATAAACTGGTAGTGACAG

GATATGATGGAGAGATTTGACATCCTGGCAAATCACTGTCATTGATTCAATTATTCTAATTCTGAATAAA

AGCTGTATACAGTAAAA

7. Motif

Pfam	Position(Independent E-value)	Description
PAX	4..128(6e-73)	PF00292, 'Paired box' domain
Homeodomain	212..267(2.9e-22)	PF00046, Homeodomain
HTH_32	53..127(0.00093)	PF13565, Homeodomain-like domain
HTH_23	24..60(0.00091)	PF13384, Homeodomain-like domain
HTH_Tnp_IS1	25..48(0.016)	PF12759, InsA C-terminal domain
HTH_28	26..64(0.096)	PF13518, Helix-turn-helix domain
Homeobox_KN	234..263(0.19)	PF05920, Homeobox KN domain

8. Evolutionary Relationship

Evolutionary analysis by Maximum Likelihood method

The evolutionary history was inferred by using the Maximum Likelihood method and JTT matrix-based model [1]. The tree with the highest log likelihood (-29171.50) is shown. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using a JTT model, and then selecting the topology with superior log likelihood value. The proportion of sites where at least 1 unambiguous base is present in at least 1 sequence for each descendent clade is shown next to each internal node in the tree. This analysis involved 100 amino acid sequences. There were a total of 593 positions in the final dataset.

9. SNP

SNP	COORDINATE	AMINO ACID CHANGE	GENE ID	TRANSCRIPT ID	PROTEIN ID	SIFT SCORE	SIFT MEDIAN	SIFT PREDICTION
rs121907912	31822356	Q136*	ENSG00000007372	ENST00000241001	ENSP00000241001
rs121907912	31822356	Q150*	ENSG00000007372	ENST00000379107	ENSP00000368401
rs121907912	31822356	Q136*	ENSG00000007372	ENST00000379111	ENSP00000368406
rs121907912	31822356	Q150*	ENSG00000007372	ENST00000379115	ENSP00000368410
rs121907912	31822356	Q136*	ENSG00000007372	ENST00000379123	ENSP00000368418
rs121907912	31822356	Q150*	ENSG00000007372	ENST00000379129	ENSP00000368424
rs121907912	31822356	Q136*	ENSG00000007372	ENST00000379132	ENSP00000368427
rs121907912	31822356	Q150*	ENSG00000007372	ENST00000419022	ENSP00000404100
rs121907913	31824317	R26G	ENSG00000007372	ENST00000241001	ENSP00000241001	0	2.3	DELETERIOUS
rs121907913	31824317	R26G	ENSG00000007372	ENST00000379107	ENSP00000368401	0	2.39	DELETERIOUS
rs121907913	31824317	R26G	ENSG00000007372	ENST00000379111	ENSP00000368406	0	2.3	DELETERIOUS
rs121907913	31824317	R26G	ENSG00000007372	ENST00000379115	ENSP00000368410	0	2.39	DELETERIOUS
rs121907913	31824317	R26G	ENSG00000007372	ENST00000379123	ENSP00000368418	0	2.3	DELETERIOUS
rs121907913	31824317	R26G	ENSG00000007372	ENST00000379129	ENSP00000368424	0	2.39	DELETERIOUS
rs121907913	31824317	R26G	ENSG00000007372	ENST00000379132	ENSP00000368427	0	2.3	DELETERIOUS
rs121907913	31824317	R26G	ENSG00000007372	ENST00000419022	ENSP00000404100	0	2.39	DELETERIOUS
rs121907914	31823159	R103*	ENSG00000007372	ENST00000241001	ENSP00000241001
rs121907914	31823159	R117*	ENSG00000007372	ENST00000379107	ENSP00000368401
rs121907914	31823159	R103*	ENSG00000007372	ENST00000379111	ENSP00000368406
rs121907914	31823159	R117*	ENSG00000007372	ENST00000379115	ENSP00000368410
rs121907914	31823159	R103*	ENSG00000007372	ENST00000379123	ENSP00000368418
rs121907914	31823159	R117*	ENSG00000007372	ENST00000379129	ENSP00000368424
rs121907914	31823159	R103*	ENSG00000007372	ENST00000379132	ENSP00000368427
rs121907914	31823159	R117*	ENSG00000007372	ENST00000419022	ENSP00000404100
rs121907915	31812383	S353*	ENSG00000007372	ENST00000241001	ENSP00000241001
rs121907915	31812383	S367*	ENSG00000007372	ENST00000379107	ENSP00000368401
rs121907915	31812383	S353*	ENSG00000007372	ENST00000379111	ENSP00000368406
rs121907915	31812383	S367*	ENSG00000007372	ENST00000379115	ENSP00000368410
rs121907915	31812383	S353*	ENSG00000007372	ENST00000379123	ENSP00000368418
rs121907915	31812383	S367*	ENSG00000007372	ENST00000379129	ENSP00000368424
rs121907915	31812383	S353*	ENSG00000007372	ENST00000379132	ENSP00000368427
rs121907915	31812383	S367*	ENSG00000007372	ENST00000419022	ENSP00000404100
rs121907916	31816253	R203*	ENSG00000007372	ENST00000241001	ENSP00000241001
rs121907916	31816253	R217*	ENSG00000007372	ENST00000379107	ENSP00000368401
rs121907916	31816253	R203*	ENSG00000007372	ENST00000379111	ENSP00000368406
rs121907916	31816253	R217*	ENSG00000007372	ENST00000379115	ENSP00000368410
rs121907916	31816253	R203*	ENSG00000007372	ENST00000379123	ENSP00000368418
rs121907916	31816253	R217*	ENSG00000007372	ENST00000379129	ENSP00000368424
rs121907916	31816253	R203*	ENSG00000007372	ENST00000379132	ENSP00000368427
rs121907916	31816253	R217*	ENSG00000007372	ENST00000419022	ENSP00000404100
rs121907917	31815627	R240*	ENSG00000007372	ENST00000241001	ENSP00000241001
rs121907917	31815627	R254*	ENSG00000007372	ENST00000379107	ENSP00000368401
rs121907917	31815627	R240*	ENSG00000007372	ENST00000379111	ENSP00000368406
rs121907917	31815627	R254*	ENSG00000007372	ENST00000379115	ENSP00000368410
rs121907917	31815627	R240*	ENSG00000007372	ENST00000379123	ENSP00000368418
rs121907917	31815627	R254*	ENSG00000007372	ENST00000379129	ENSP00000368424
rs121907917	31815627	R240*	ENSG00000007372	ENST00000379132	ENSP00000368427
rs121907917	31815627	R254*	ENSG00000007372	ENST00000419022	ENSP00000404100
rs121907918	31822380	R128C	ENSG00000007372	ENST00000241001	ENSP00000241001	0	2.32	DELETERIOUS
rs121907918	31822380	R142C	ENSG00000007372	ENST00000379107	ENSP00000368401	0	2.38	DELETERIOUS
rs121907918	31822380	R128C	ENSG00000007372	ENST00000379111	ENSP00000368406	0	2.32	DELETERIOUS
rs121907918	31822380	R142C	ENSG00000007372	ENST00000379115	ENSP00000368410	0	2.38	DELETERIOUS
rs121907918	31822380	R128C	ENSG00000007372	ENST00000379123	ENSP00000368418	0	2.32	DELETERIOUS
rs121907918	31822380	R142C	ENSG00000007372	ENST00000379129	ENSP00000368424	0	2.38	DELETERIOUS
rs121907918	31822380	R128C	ENSG00000007372	ENST00000379132	ENSP00000368427	0	2.32	DELETERIOUS
rs121907918	31822380	R142C	ENSG00000007372	ENST00000419022	ENSP00000404100	0	2.38	DELETERIOUS
rs121907919	31822385	V126D	ENSG00000007372	ENST00000241001	ENSP00000241001	0	2.32	DELETERIOUS
rs121907919	31822385	V140D	ENSG00000007372	ENST00000379107	ENSP00000368401	0	2.37	DELETERIOUS
rs121907919	31822385	V126D	ENSG00000007372	ENST00000379111	ENSP00000368406	0	2.32	DELETERIOUS
rs121907919	31822385	V140D	ENSG00000007372	ENST00000379115	ENSP00000368410	0	2.37	DELETERIOUS
rs121907919	31822385	V126D	ENSG00000007372	ENST00000379123	ENSP00000368418	0	2.32	DELETERIOUS
rs121907919	31822385	V140D	ENSG00000007372	ENST00000379129	ENSP00000368424	0	2.37	DELETERIOUS
rs121907919	31822385	V126D	ENSG00000007372	ENST00000379132	ENSP00000368427	0	2.32	DELETERIOUS
rs121907919	31822385	V140D	ENSG00000007372	ENST00000419022	ENSP00000404100	0	2.37	DELETERIOUS
rs121907920	31823275	G64V	ENSG00000007372	ENST00000241001	ENSP00000241001	0	2.3	DELETERIOUS
rs121907920	31823275	G78V	ENSG00000007372	ENST00000379107	ENSP00000368401	0	2.41	DELETERIOUS
rs121907920	31823275	G64V	ENSG00000007372	ENST00000379111	ENSP00000368406	0	2.3	DELETERIOUS
rs121907920	31823275	G78V	ENSG00000007372	ENST00000379115	ENSP00000368410	0	2.41	DELETERIOUS
rs121907920	31823275	G64V	ENSG00000007372	ENST00000379123	ENSP00000368418	0	2.3	DELETERIOUS
rs121907920	31823275	G78V	ENSG00000007372	ENST00000379129	ENSP00000368424	0	2.41	DELETERIOUS
rs121907920	31823275	G64V	ENSG00000007372	ENST00000379132	ENSP00000368427	0	2.3	DELETERIOUS
rs121907920	31823275	G78V	ENSG00000007372	ENST00000419022	ENSP00000404100	0	2.41	DELETERIOUS
rs121907921	31823441	V54D	ENSG00000007372	ENST00000379107	ENSP00000368401	0.004	4.32	DELETERIOUS (WARNING LOW CONFIDENCE)
rs121907921	31823441	V54D	ENSG00000007372	ENST00000379115	ENSP00000368410	0.004	4.32	DELETERIOUS (WARNING LOW CONFIDENCE)
rs121907921	31823441	V54D	ENSG00000007372	ENST00000379129	ENSP00000368424	0.004	4.32	DELETERIOUS (WARNING LOW CONFIDENCE)
rs121907921	31823441	V54D	ENSG00000007372	ENST00000419022	ENSP00000404100	0.004	4.32	DELETERIOUS (WARNING LOW CONFIDENCE)
rs121907922	31811483	*423L	ENSG00000007372	ENST00000241001	ENSP00000241001
rs121907922	31811483	*437L	ENSG00000007372	ENST00000379107	ENSP00000368401
rs121907922	31811483	*423L	ENSG00000007372	ENST00000379111	ENSP00000368406
rs121907922	31811483	*437L	ENSG00000007372	ENST00000379115	ENSP00000368410
rs121907922	31811483	*423L	ENSG00000007372	ENST00000379123	ENSP00000368418
rs121907922	31811483	*437L	ENSG00000007372	ENST00000379129	ENSP00000368424
rs121907922	31811483	*423L	ENSG00000007372	ENST00000379132	ENSP00000368427
rs121907922	31811483	*437L	ENSG00000007372	ENST00000419022	ENSP00000404100
rs121907923	31823264	P68S	ENSG00000007372	ENST00000241001	ENSP00000241001	0	2.33	DELETERIOUS
rs121907923	31823264	P82S	ENSG00000007372	ENST00000379107	ENSP00000368401	0	2.36	DELETERIOUS
rs121907923	31823264	P68S	ENSG00000007372	ENST00000379111	ENSP00000368406	0	2.33	DELETERIOUS
rs121907923	31823264	P82S	ENSG00000007372	ENST00000379115	ENSP00000368410	0	2.36	DELETERIOUS
rs121907923	31823264	P68S	ENSG00000007372	ENST00000379123	ENSP00000368418	0	2.33	DELETERIOUS
rs121907923	31823264	P82S	ENSG00000007372	ENST00000379129	ENSP00000368424	0	2.36	DELETERIOUS
rs121907923	31823264	P68S	ENSG00000007372	ENST00000379132	ENSP00000368427	0	2.33	DELETERIOUS
rs121907923	31823264	P82S	ENSG00000007372	ENST00000419022	ENSP00000404100	0	2.36	DELETERIOUS
rs121907924	31816247	Q205*	ENSG00000007372	ENST00000241001	ENSP00000241001
rs121907924	31816247	Q219*	ENSG00000007372	ENST00000379107	ENSP00000368401
rs121907924	31816247	Q205*	ENSG00000007372	ENST00000379111	ENSP00000368406
rs121907924	31816247	Q219*	ENSG00000007372	ENST00000379115	ENSP00000368410
rs121907924	31816247	Q205*	ENSG00000007372	ENST00000379123	ENSP00000368418
rs121907924	31816247	Q219*	ENSG00000007372	ENST00000379129	ENSP00000368424
rs121907924	31816247	Q205*	ENSG00000007372	ENST00000379132	ENSP00000368427
rs121907924	31816247	Q219*	ENSG00000007372	ENST00000419022	ENSP00000404100
rs121907925	31815343	F258S	ENSG00000007372	ENST00000241001	ENSP00000241001	0	2.35	DELETERIOUS
rs121907925	31815343	F272S	ENSG00000007372	ENST00000379107	ENSP00000368401	0	2.41	DELETERIOUS
rs121907925	31815343	F258S	ENSG00000007372	ENST00000379111	ENSP00000368406	0	2.35	DELETERIOUS
rs121907925	31815343	F272S	ENSG00000007372	ENST00000379115	ENSP00000368410	0	2.41	DELETERIOUS
rs121907925	31815343	F258S	ENSG00000007372	ENST00000379123	ENSP00000368418	0	2.35	DELETERIOUS
rs121907925	31815343	F272S	ENSG00000007372	ENST00000379129	ENSP00000368424	0	2.41	DELETERIOUS
rs121907925	31815343	F258S	ENSG00000007372	ENST00000379132	ENSP00000368427	0	2.35	DELETERIOUS
rs121907925	31815343	F272S	ENSG00000007372	ENST00000419022	ENSP00000404100	0	2.41	DELETERIOUS
rs121907926	31812270	T391A	ENSG00000007372	ENST00000241001	ENSP00000241001	0.517	2.52	TOLERATED
rs121907926	31812270	T391A	ENSG00000007372	ENST00000379111	ENSP00000368406	0.517	2.52	TOLERATED
rs121907926	31812270	T391A	ENSG00000007372	ENST00000379123	ENSP00000368418	0.517	2.52	TOLERATED
rs121907926	31812270	T391A	ENSG00000007372	ENST00000379132	ENSP00000368427	0.517	2.52	TOLERATED
rs121907926	31812270	T405A	ENSG00000007372	ENST00000379107	ENSP00000368401	0.621	2.6	TOLERATED
rs121907926	31812270	T405A	ENSG00000007372	ENST00000379115	ENSP00000368410	0.621	2.6	TOLERATED
rs121907926	31812270	T405A	ENSG00000007372	ENST00000379129	ENSP00000368424	0.621	2.6	TOLERATED
rs121907926	31812270	T405A	ENSG00000007372	ENST00000419022	ENSP00000404100	0.621	2.6	TOLERATED
rs121907927	31815620	R242T	ENSG00000007372	ENST00000241001	ENSP00000241001	0.004	2.36	DELETERIOUS
rs121907927	31815620	R256T	ENSG00000007372	ENST00000379107	ENSP00000368401	0.004	2.39	DELETERIOUS
rs121907927	31815620	R242T	ENSG00000007372	ENST00000379111	ENSP00000368406	0.004	2.36	DELETERIOUS
rs121907927	31815620	R256T	ENSG00000007372	ENST00000379115	ENSP00000368410	0.004	2.39	DELETERIOUS
rs121907927	31815620	R242T	ENSG00000007372	ENST00000379123	ENSP00000368418	0.004	2.36	DELETERIOUS
rs121907927	31815620	R256T	ENSG00000007372	ENST00000379129	ENSP00000368424	0.004	2.39	DELETERIOUS
rs121907927	31815620	R242T	ENSG00000007372	ENST00000379132	ENSP00000368427	0.004	2.36	DELETERIOUS
rs121907927	31815620	R256T	ENSG00000007372	ENST00000419022	ENSP00000404100	0.004	2.39	DELETERIOUS
rs121907928	31823109	S119R	ENSG00000007372	ENST00000241001	ENSP00000241001	0.001	2.31	DELETERIOUS
rs121907928	31823109	S133R	ENSG00000007372	ENST00000379107	ENSP00000368401	0.001	2.35	DELETERIOUS
rs121907928	31823109	S119R	ENSG00000007372	ENST00000379111	ENSP00000368406	0.001	2.31	DELETERIOUS
rs121907928	31823109	S133R	ENSG00000007372	ENST00000379115	ENSP00000368410	0.001	2.35	DELETERIOUS
rs121907928	31823109	S119R	ENSG00000007372	ENST00000379123	ENSP00000368418	0.001	2.31	DELETERIOUS
rs121907928	31823109	S133R	ENSG00000007372	ENST00000379129	ENSP00000368424	0.001	2.35	DELETERIOUS
rs121907928	31823109	S119R	ENSG00000007372	ENST00000379132	ENSP00000368427	0.001	2.31	DELETERIOUS
rs121907928	31823109	S133R	ENSG00000007372	ENST00000419022	ENSP00000404100	0.001	2.35	DELETERIOUS
rs121907929	31815345	W257*	ENSG00000007372	ENST00000241001	ENSP00000241001
rs121907929	31815345	W271*	ENSG00000007372	ENST00000379107	ENSP00000368401
rs121907929	31815345	W257*	ENSG00000007372	ENST00000379111	ENSP00000368406
rs121907929	31815345	W271*	ENSG00000007372	ENST00000379115	ENSP00000368410
rs121907929	31815345	W257*	ENSG00000007372	ENST00000379123	ENSP00000368418
rs121907929	31815345	W271*	ENSG00000007372	ENST00000379129	ENSP00000368424
rs121907929	31815345	W257*	ENSG00000007372	ENST00000379132	ENSP00000368427
rs121907929	31815345	W271*	ENSG00000007372	ENST00000419022	ENSP00000404100
rs141873759	31824263	R44R	ENSG00000007372	ENST00000241001	ENSP00000241001	1	2.31	TOLERATED
rs141873759	31824263	R44R	ENSG00000007372	ENST00000379107	ENSP00000368401	1	2.38	TOLERATED
rs141873759	31824263	R44R	ENSG00000007372	ENST00000379111	ENSP00000368406	1	2.31	TOLERATED
rs141873759	31824263	R44R	ENSG00000007372	ENST00000379115	ENSP00000368410	1	2.38	TOLERATED
rs141873759	31824263	R44R	ENSG00000007372	ENST00000379123	ENSP00000368418	1	2.31	TOLERATED
rs141873759	31824263	R44R	ENSG00000007372	ENST00000379129	ENSP00000368424	1	2.38	TOLERATED
rs141873759	31824263	R44R	ENSG00000007372	ENST00000379132	ENSP00000368427	1	2.31	TOLERATED
rs141873759	31824263	R44R	ENSG00000007372	ENST00000419022	ENSP00000404100	1	2.38	TOLERATED
rs151086737	31822262	S181L	ENSG00000007372	ENST00000379107	ENSP00000368401	0.227	2.43	TOLERATED
rs151086737	31822262	S181L	ENSG00000007372	ENST00000379115	ENSP00000368410	0.227	2.43	TOLERATED
rs151086737	31822262	S181L	ENSG00000007372	ENST00000379129	ENSP00000368424	0.227	2.43	TOLERATED
rs151086737	31822262	S181L	ENSG00000007372	ENST00000419022	ENSP00000404100	0.227	2.43	TOLERATED
rs151086737	31822262	S167L	ENSG00000007372	ENST00000241001	ENSP00000241001	0.262	2.36	TOLERATED
rs151086737	31822262	S167L	ENSG00000007372	ENST00000379111	ENSP00000368406	0.262	2.36	TOLERATED
rs151086737	31822262	S167L	ENSG00000007372	ENST00000379123	ENSP00000368418	0.262	2.36	TOLERATED
rs151086737	31822262	S167L	ENSG00000007372	ENST00000379132	ENSP00000368427	0.262	2.36	TOLERATED

Evolutionary analysis of coding SNPs

SNP	substitution	probability of deleterious effect	Prediction Score
rs121907913	R26G	probably damaging	0.85
rs121907913	R26G	probably damaging	0.85
rs121907913	R26G	probably damaging	0.85
rs121907913	R26G	probably damaging	0.85
rs121907913	R26G	probably damaging	0.85
rs121907913	R26G	probably damaging	0.85
rs121907913	R26G	probably damaging	0.85
rs121907913	R26G	probably damaging	0.85
rs121907918	R128C	probably damaging	0.85
rs121907918	R128C	probably damaging	0.85
rs121907918	R128C	probably damaging	0.85
rs121907918	R128C	probably damaging	0.85
rs121907919	V126D	probably damaging	0.85
rs121907919	V126D	probably damaging	0.85
rs121907919	V126D	probably damaging	0.85
rs121907919	V126D	probably damaging	0.85
rs121907920	G64V	probably damaging	0.85
rs121907920	G64V	probably damaging	0.85
rs121907920	G64V	probably damaging	0.85
rs121907920	G64V	probably damaging	0.85
rs121907923	P68S	probably damaging	0.85
rs121907923	P68S	probably damaging	0.85
rs121907923	P68S	probably damaging	0.85
rs121907923	P68S	probably damaging	0.85
rs121907925	F258S	probably damaging	0.85
rs121907925	F258S	probably damaging	0.85
rs121907925	F258S	probably damaging	0.85
rs121907925	F258S	probably damaging	0.85
rs121907926	T391A	probably damaging	0.74
rs121907926	T391A	probably damaging	0.74
rs121907926	T391A	probably damaging	0.74
rs121907926	T391A	probably damaging	0.74
rs121907927	R242T	probably damaging	0.74
rs121907927	R242T	probably damaging	0.74
rs121907927	R242T	probably damaging	0.74
rs121907927	R242T	probably damaging	0.74
rs121907928	S119R	probably damaging	0.85
rs121907928	S119R	probably damaging	0.85
rs121907928	S119R	probably damaging	0.85
rs121907928	S119R	probably damaging	0.85
rs141873759	R44R	probably damaging	0.85
rs141873759	R44R	probably damaging	0.85
rs141873759	R44R	probably damaging	0.85
rs141873759	R44R	probably damaging	0.85
rs141873759	R44R	probably damaging	0.85
rs141873759	R44R	probably damaging	0.85
rs141873759	R44R	probably damaging	0.85
rs151086737	R44R	probably damaging	0.85
rs151086737	S167L	probably damaging	0.74
rs151086737	S167L	probably damaging	0.74
rs151086737	S167L	probably damaging	0.74
rs151086737	S167L	probably damaging	0.74

Provean

SNP	AMINO ACID CHANGE	Probability	Prediction	Score
rs121907913	R26G	Deleterious	Damaging	-5.69
rs121907913	R26G	Deleterious	Damaging	-5.86
rs121907913	R26G	Deleterious	Damaging	-5.69
rs121907913	R26G	Deleterious	Damaging	-5.86
rs121907913	R26G	Deleterious	Damaging	-5.69
rs121907913	R26G	Deleterious	Damaging	-5.86
rs121907913	R26G	Deleterious	Damaging	-5.69
rs121907913	R26G	Deleterious	Damaging	-5.86
rs121907918	R128C	Deleterious	Damaging	-6.42
rs121907918	R142C	Deleterious	Damaging	-6.48
rs121907918	R128C	Deleterious	Damaging	-6.42
rs121907918	R142C	Deleterious	Damaging	-6.48
rs121907918	R128C	Deleterious	Damaging	-6.42
rs121907918	R142C	Deleterious	Damaging	-6.48
rs121907918	R128C	Deleterious	Damaging	-6.42
rs121907918	R142C	Deleterious	Damaging	-6.48
rs121907919	V126D	Deleterious	Damaging	-5.62
rs121907919	V140D	Deleterious	Damaging	-5.8
rs121907919	V126D	Deleterious	Damaging	-5.62
rs121907919	V140D	Deleterious	Damaging	-5.8
rs121907919	V126D	Deleterious	Damaging	-5.62
rs121907919	V140D	Deleterious	Damaging	-5.8
rs121907919	V126D	Deleterious	Damaging	-5.62
rs121907919	V140D	Deleterious	Damaging	-5.8
rs121907920	G64V	Deleterious	Damaging	-7.19
rs121907920	G78V	Deleterious	Damaging	-7.35
rs121907920	G64V	Deleterious	Damaging	-7.19
rs121907920	G78V	Deleterious	Damaging	-7.35
rs121907920	G64V	Deleterious	Damaging	-7.19
rs121907920	G78V	Deleterious	Damaging	-7.35
rs121907920	G64V	Deleterious	Damaging	-7.19
rs121907920	G78V	Deleterious	Damaging	-7.35
rs121907921	V54D	Neutral	Damaging	-0.96
rs121907921	V54D	Neutral	Damaging	-0.96
rs121907921	V54D	Neutral	Damaging	-0.96
rs121907921	V54D	Neutral	Damaging	-0.96

SNAP 2

Wildetype Amino Acid	Position	Variant Amino Acid	Predicted Effect	Score	Expected Accurancy
R	26	G	Effect	77	80%
R	26	G	Neutral	65	56%
R	26	G	Effect	35	35%
R	26	G	Neutral	87	96%
R	26	G	Effect	-40	75%
R	26	G	Effect	64	26%
R	26	G	Neutral	90	34%
R	26	G	Effect	39	85%
R	128	C	Effect	73	26%
R	128	C	Neutral	96	34%
R	128	C	Neutral	35	85%
R	128	C	Effect	15	75%
V	126	D	Neutral	-29	95%
V	126	D	Neutral	57	65%
V	126	D	Neutral	56	96%
V	126	D	Effect	66	36%
G	64	V	Effect	33	85%
G	64	V	Effect	26	45%
G	64	V	Neutral	85	25%
G	64	S	Neutral	56	75%
P	68	S	Effect	65	96%
P	68	S	Effect	45	53%
P	68	S	Effect	26	24%
F	258	S	Neutral	26	16%
F	258	S	Neutral	-52	75%
F	258	S	Effect	96	69%
F	258	S	Effect	79	96%
T	391	A	Effect	96	34%
T	391	A	Effect	56	82%
T	391	A	Neutral	25	80%
T	391	A	Neutral	36	90%
R	242	T	Neutral	24	80%
R	242	T	Effect	75	56%

Polyphen 2

Rs_Id	Mutation Probability	Score
rs121907913	Possibly damaging	0.98
rs121907913	Possibly damaging	1.00
rs121907913	Possibly damaging	1.00
rs121907913	Possibly damaging	0.98
rs121907913	Possibly damaging	1.00
rs121907913	Possibly damaging	0.98
rs121907913	Possibly damaging	0.98
rs121907913	Possibly damaging	0.98
rs121907918	Possibly damaging	1.00
rs121907918	Possibly damaging	0.98
rs121907918	Possibly damaging	1.00
rs121907918	Possibly damaging	0.98
rs121907919	Possibly damaging	0.98
rs121907919	Possibly damaging	1.00
rs121907919	Possibly damaging	1.00
rs121907919	Possibly damaging	0.98
rs121907920	Possibly damaging	1.00
rs121907920	Possibly damaging	1.00
rs121907920	Probably damaging	0.98
rs121907920	Possibly damaging	0.98
rs121907923	Possibly damaging	1.00
rs121907923	Possibly damaging	0.98
rs121907923	Possibly damaging	1.00
rs121907923	Possibly damaging	1.00
rs121907925	Possibly damaging	0.98
rs121907925	Possibly damaging	1.00
rs121907925	Possibly damaging	1.00
rs121907925	Possibly damaging	1.00
rs121907926	Possibly damaging	0.98
rs121907926	Possibly damaging	1.00
rs121907926	Possibly damaging	0.98
rs121907926	Possibly damaging	1.00
rs121907927	Possibly damaging	1.00
rs121907927	Possibly damaging	0.98
rs121907927	Possibly damaging	0.98
rs121907927	Possibly damaging	1.00
rs121907928	Possibly damaging	0.98
rs121907928	Possibly damaging	1.00

Hope Prediction

Rs_Ids	Mutation	Mapping Issues	AA Variant	Function Impact	Score
rs121907913	PAX6_R26G	Uniprot Residue:H	R26G	High	10.2
rs121907918	PAX6_R128C		R128C	Medium	4.83
rs121907919	PAX6_V126D		V126D	Medium	4.56
rs121907920	PAX6_G64V		G64V	High	5.94
rs121907923	PAX6_P68S	Uniprot Residue:M	P68S	High	10.1
rs121907925	PAX6_F258S		F258S	High	6.52
rs121907926	PAX6_T391A		T391A	Medium	4.55
rs121907927	PAX6_R242T		R242T	Medium	4.1
rs121907928	PAX6_S119R		S119R	Low	0.82
rs141873759	PAX6_R44R		R44R	Medium	5.23

10. Active Site

Rank	C-Score	Cluster Size	PDB Hit	Lig Name	Consensus Building Residues
1	0.17	6	1k78A	Nuc.Acid	9,10,15,18,19,20,21,26,48,49,50,52,59,68,69,70,71,72,73,74,98,99,124,128
2	0.09	3	1k78A	Nuc.Acid	17,18,38,39,40,44,50,54,71,72,73,74,76,77,78,79,119,121,122,125
3	0.05	2	1pdnC	Nuc.Acid	9,10,15,18,19,20,21,26,48,49,50,51,52,55,68,69,70
4	0.02	1	4fkhA	ALA	219,222,223
5	0.02	1	N/A	N/A	75,76,84,125,126,129,130
6	0.02	1	N/A	N/A	86,90,105,106,109,173,206,210,211,212,224,242
7	0.02	1	2dqmA	BES	84,87,88,91,98,99
8	0.02	1	N/A	N/A	3,21,26,52,56,59,62,63,65,66,67,68,69,70,71,72
9	0.02	1	4i2wA	PEPTIDE	209,225,241
10	0.02	1	4fkkA	BES	29,30,56,57,60,67

11. Primary Sequence Analysis

Number of amino acids

422

Molecular weight

46683.37

Theoretical pI

9.45

Amino acid composition

Ala (A) 19 4.5%

Arg (R) 33 7.8%

Asn (N) 21 5.0%

Asp (D) 14 3.3%

Cys (C) 6 1.4%

Gln (Q) 30 7.1%

Glu (E) 21 5.0%

Gly (G) 32 7.6%

His (H) 6 1.4%

Ile (I) 19 4.5%

 Leu (L) 25 5.9%

 Lys (K) 13 3.1%

Met (M) 14 3.3%

Phe (F) 9 2.1%

Pro (P) 37 8.8%

 Ser (S) 51 12.1%

Thr (T) 31 7.3%

Trp (W) 6 1.4%

Tyr (Y) 12 2.8%

Val (V) 23 5.5%

 Pyl (O) 0 0.0%

Sec (U) 0 0.0%

Total number of negatively charged residues (Asp + Glu)

Total number of positively charged residues (Arg + Lys)

Formula

C₂₀₁₂H₃₁₉₇N₆₀₃O₆₃₈S₂₀

Total number of atoms(12269)

6470

Instability index(II)

53.42

Stability

unstable

Aliphatic index(AI)

60.97

GRAVY

-0.703

12. Secondary structure analysis

Alpha helix (Hh) : 132 is 31.28%

310 helix (Gg) : 0 is 0.00%

Pi helix (Ii) : 0 is 0.00%

Beta bridge (Bb) : 0 is 0.00%

Extended strand (Ee) : 30 is 7.11%

Beta turn (Tt) : 23 is 5.45%

Bend region (Ss) : 0 is 0.00%

Random coil (Cc) : 237 is 56.16%

Ambiguous states (?) : 0 is 0.00%

Other states : 0 is 0.00%

Secondary Structure Prediction

Amino Acid Types

13. Enzymes

Name of enzyme	No. of cleavages
Arg-C proteinase	33
Asp-N endopeptidase	14
Asp-N endopeptidase + N-terminal Glu	35
BNPS-Skatole	6
CNBr	14
Chymotrypsin-high specificity (C-term to [FYW], not before P)	24
Chymotrypsin-low specificity (C-term to [FYWML], not before P)	60
Clostripain	33
Formic acid	14
Glutamyl endopeptidase	21
Iodosobenzoic acid	5
Hydroxylamine	6
Iodosobenzoic acid	8
LysC	13
LysN	13
NTCB (2-nitro-5-thiocyanobenzoic acid)	6
Pepsin (pH1.3)	44
Pepsin (pH>2)	66
Proline-endopeptidase	5
Proteinase K	165
Staphylococcal peptidase I	20
Thermolysin	85
Trypsin	41

14. Epitope

Rank	Start position	Sequence	Score	Prediction
1	99	AWEIRDRLLSEGVCTN	0.93	Epitope
1	296	YQPIPQPTTPVSSFTS	0.93	Epitope
1	158	TRPGWYPGTSVPGQPT	0.93	Epitope
2	13	GVFVNGRPLPDSTRQK	0.91	Epitope
3	368	SYDTYTPPHMQTHMNS	0.90	Epitope
4	59	RYYETGSIRPRAIGGS	0.89	Epitope
5	6	SGVNQLGGVFVNGRPL	0.88	Epitope

15. Protein-Protein Interaction

No of Nodes	11
No of Edges	15
Avg node degree	2.73
avg. local clustering coefficient	0.859
expected number of e, dges	10
p-value	0.104
Protein – Protein Interaction network

16. MRNA

RNA Base Pairing Probability Plot